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Record W7132995388

Non-stick surfaces for digital microfluidics

2008· dissertation· W7132995388 on OpenAlexaff
Vivienne Nancy Luk

Bibliographic record

VenueTSpace · 2008
Typedissertation
Language
FieldEngineering
TopicElectrowetting and Microfluidic Technologies
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsMicrofluidicsSubstrate (aquarium)AdsorptionProtein adsorptionMass spectrometryReagentDigital microfluidics
DOInot available

Abstract

fetched live from OpenAlex

Digital microfluidics (DMF) is a promising technique for carrying out miniaturized, automated biochemical assays, where discrete droplets of reagents are actuated on the surface of an array of electrodes. A limitation for DMF is non-specific protein adsorption to device surfaces, which interferes with assay fidelity and can cause mechanical problems. A quantitative analysis of protein adsorption on DMF devices by means of confocal microscopy and secondary ion mass spectrometry are presented. This study resulted in a simple and effective method for preventing protein adsorption: the use of low concentrations of Pluronic F127 as a solution additive. This strategy facilitated the actuation of droplets containing 10,000x higher protein concentrations than is possible without the additive. A DMF-driven protein digest assay using large concentrations of protein substrate was implemented to illustrate the benefits. This technique makes non-stick surfaces in DMF a reality, which greatly expands the range of applications that are compatible with this technology.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.055
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.272
Teacher spread0.260 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

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