Listeriolysin O Induces a Phagosome Stress Response during Listeria monocytogenes Growth in Spacious Vacuoles
Bibliographic record
Abstract
Despite a traditional view of Listeria monocytogenes as a cytosolic pathogen, our group previously found that in macrophages a subset of Listeria occupies spacious vacuolar compartments. Intriguingly, this replication niche is achieved through secretion of low levels of listeriolysin O (LLO), a listerial pore forming cytolysin known to promote vacuolar escape. While cells coordinate a variety of systems for repair and removal to counteract compromised membrane integrity, spacious Listeria-containing phagosomes (SLAPs) block maturation into microbicidal phagolysosomes. The mechanisms by which LLO initiates this niche for growth in macrophages has remained unclear. My research has focused on characterizing these persistent structures and how they evade degradation. I have discovered a complex network of proteins, lipids, and lack thereof, attributing to sustained Listeria infection in SLAPs. Altogether, this characterization serves as a valuable resource for future host-pathogen studies, and emphasizes the many subversive tools developed by pathogens to ensure intracellular survival.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".