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Record W7133034184

Investigating the Role of 3-ketosphinganine Reductase (KDSR) in Acute Myeloid Leukemia

2022· dissertation· W7133034184 on OpenAlexfundno aff
Jennifer So Yeon Ahn

Bibliographic record

VenueTSpace · 2022
Typedissertation
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicSphingolipid Metabolism and Signaling
Canadian institutionsnot available
FundersUniversity of Toronto
KeywordsSphingolipidMyeloid leukemiaGene knockdownLeukemiaBone marrowPathogenesisCellDisease
DOInot available

Abstract

fetched live from OpenAlex

AML is characterized by accumulation of poorly differentiated, non-functional myeloblasts in the bone marrow and blood. Despite therapeutic advancements, AML remains a disease with poor long-term survival rates. To develop improved therapeutic strategies, this study aims to understand and target sphingolipid metabolism in AML. Sphingolipids are a heterogenous class of lipids that share an 18-carbon amino alcohol backbone. Bioactive sphingolipids regulate leukemic cell survival, apoptosis, chemoresistance, and differentiation. In this study, we show that early sphingolipid synthesis enzyme, 3-ketosphinganine reductase (KDSR), is essential for AML pathogenesis. KDSR is overexpressed in primary AML patient samples compared to healthy controls. Knockdown of KDSR impairs proliferation, viability, and self-renewal properties in AML cell lines. Using liquid-chromatography mass-spectrometry, we show that lack of KDSR activity induces a significant change in the cellular lipidome. Altogether, our findings suggest that KDSR promotes AML pathogenesis by regulating lipid homeostasis, thereby proposing KDSR as a novel target in AML.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.284
Teacher spread0.276 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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