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Record W7133051306

Inhibition of muscle cell osteodifferentiation by fetuin

2004· dissertation· W7133051306 on OpenAlexaff
Samuel Isaac Barkin

Bibliographic record

VenueTSpace · 2004
Typedissertation
Language
FieldMedicine
TopicParathyroid Disorders and Treatments
Canadian institutionsBibliographical Society of CanadaUniversity of Toronto
Fundersnot available
KeywordsFetuinC2C12MyocyteBone morphogenetic proteinMesenchymal stem cellPathophysiologySkeletal muscleCell
DOInot available

Abstract

fetched live from OpenAlex

Although the pathophysiology of HO is not well understood, it has been suggested that mesenchymal cells near surgical sites, are exposed to bone particles, containing bone morphogenetic proteins (BMP). Fetuin, also known as alpha2HS-glycoprotein (Ahsg), is a serum glycoprotein, which binds TGFbeta and BMP cytokines due its BMP/TGFbeta receptor-like region within the fetuin structure. We hypothesize that the osteodifferentiation of C2C12 cells under the influence of BMPs will be inhibited by fetuin. Mouse myoblasts were grown with BMP and fetuin added to the media. There was an increase AP activity in the presence of increasing doses of BMP/NCP or rhBMP-4. Fetuin inhibited induced increases in AP in a dose-dependent fashion with the most effective dose being in the range of 50--80muM fetuin. C2C12 cultures exhibited increased CK expression with the addition of 20ng/ml of rBMP-4. This increase was similarly inhibited by approximately 50muM fetuin. Using this model, fetuin has been shown to inhibit the effects of BMPs on target cells. As well, using this model we were able to approximate the doses of fetuin that might be required for regulation of BMP effects in vivo.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.302
Teacher spread0.290 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2004
Admission routes1
Has abstractyes

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