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Record W7135260474

Immuno-pathophysiological role of miR-23b and ROS in sepsis in newborns

2021· dissertation· fr· W7135260474 on OpenAlexfundno aff
Fatmi Ahlam

Bibliographic record

VenueDépôt Institutionnel de lUniversité de Tlemcen · 2021
Typedissertation
Languagefr
FieldMedicine
TopicNeonatal and Maternal Infections
Canadian institutionsnot available
FundersGrand Challenges Canada
KeywordsSepsisNeonatal sepsisOxidative stressReactive oxygen speciesBacteriaBetamethasoneProspective cohort studyStaphylococcus aureus
DOInot available

Abstract

fetched live from OpenAlex

bstract Background: Neonatal sepsis represent a major challenge in public health, where is the third most common cause of death of newborns. Neonatal sepsis characterized by misunderstood either at the molecular or at the cellular level. Here, in our study, we investigated the role of miRNA-23b in neonatal sepsis, and we studied the effect of reactive oxygen species in the activity of endothelial cells in sepsis. Methods: Our study has two main parts. A prospective study based on Fifty-four newborns suspected sepsis. By RT-qPCR, we quantified the level of miRNA-23b in hemoculture. Via an ex-vivo model, we used the umbilical cord, which's divided into four groups. Where the exposure to oxidative stress caused by CuSO4 and sepsis by Staphylococcus aureus. After 1 hour of incubation, we isolated endothelial cells and studied them. Results: our study showing the relationship between sepsis and miRNA-23b, where We have shown that miRNA-23b levels increased in premature and full-term newborns in the case of EOS (p < 0.001 and p < 0.005 respectively), but decreased in LOS (p < 0.005). And proved the negative correlation between newborns who died from sepsis and miRNA-23b level. In the ex-vivo model, we have shown endothelial cells behaved differently against bacteria in the four conditions With betamethasone and without CuSO4, we noted the translocation of bacteria to the blood with a decrease in the level of miR-23b. Injection of CuSO4 into the blood induces a change in the activity of ECs and neutralizes the level of miR-23b, contribute to the defense against the translocation of bacteria in the blood. Conclusions: The decrease in miRNA-23b levels would undoubtedly be an important factor favoring the development of neonatal sepsis. In addition to the protective role of copper-induced oxidative stress, activated endothelial cells promoting the pro-inflammatory response in the blood may help prevent the translocation of S. aureus to the blood. Key words: Neonatal Sepsis, Endothelial Cells, Reactive Oxygen Species (ROS), miRNA- 23b.

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How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.720
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.237
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

Explore more

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