Bibliographic record
Abstract
以美洲黑杨杂种优良无性系南林895杨（Populus×euramericana cv．‘Nanlin895’）为转基因受体材料，以嫩芽或腋芽为外植体材料组织培养再生植株，利用农杆菌介导法转化成Bt基因和CpTI基因。结果显示较合适的组培再生与遗传转化系统为：叶分化培养基为MS＋6-BA 0．5mg／L＋TDZ 0．002mg／L，芽伸长培养基为MS＋6-BA 0．2mg／L＋TDZ 0．001mg／L＋Km 10mg／L＋Carb 500mg／L，生根培养基为1／2MS；预培养3d，菌液浓度OD600 1．0-1．3左右，侵染时间20min，共培养4d，叶盘转化频率可达28．7％。对Km^r植株经PCR分析，筛选获得了18株整合有成Bt基因和1株整合有CpTI基因的转基因植株。部分转基因植株的初步饲虫实验表明，饲喂转基因杨树叶片可明显抑制杨小舟蛾的生长发育。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.007 | 0.005 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".