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Record W833259752

花粉管通道法介导il-4基因转化大白菜(Brassica pekinensis Rupr.)的研究初探

2006· article· zh· W833259752 on OpenAlexvenueno aff
王雷, 于丽杰, 凌春莹, 李然红

Bibliographic record

Venue分子植物育种 · 2006
Typearticle
Languagezh
FieldAgricultural and Biological Sciences
TopicPlant-Microbe Interactions and Immunity
Canadian institutionsnot available
Fundersnot available
KeywordsBrassicaBiologyBotany
DOInot available

Abstract

fetched live from OpenAlex

通过花粉管通道法介导il-4基因转化大白菜,利用PPT抗性筛选、PCR扩增和PCR-Southern杂交技术检测转化后代。本文针对品种、人工授粉时间和转化操作时机可能对il-4转化率产生的影响进行了研究。试验结果表明,il-4基因对品种有偏爱,哈白二号的转化率高;不同的人工授粉时期对转化率有影响,开花期授粉效果好;不同的转化时机对转化率也有影响,人工授粉后24h左右进行转化操作效果好。哈白二号在花期授粉后24h左右进行转化操作(AH),il-4的转化率达1.15%。对il-4基因在大白菜T2代中的传递规律进行观察,发现PPT的抗性分离情况,除哈白二号-2单株的后代外,其余均不符合抗性植株数:非抗性植株数=3:1的孟德尔分离规律;而il-4的分离情况,也不符合阳性植株数:阴性植株数=3:1的孟德尔分离规律。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.016

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0010.001
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.215
Teacher spread0.198 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2006
Admission routes1
Has abstractyes

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