1-AMINOCYCLOPROPANE-1-CARBOXYLATE (ACC) DEAMINASE GENES IN RHIZOBIA: ISOLATION AND CHARACTERIZATION
Bibliographic record
Abstract
A collection of 233 putative Rhizobia strains from 30 different sites across Saskatchewan, Canada was assayed for ACC deaminase activity, with 27 of the strains displaying activity. When all 27 strains were characterized based on 16S rRNA gene sequences, it was noted that 26 strains are Rhizobium leguminosarum and one strain is Rhizobium gallicum. PCR was used to rapidly isolate ACC deaminase structural genes from the above mentioned 27 strains; 17 of them have 99% identities when compared with the previously characterized ACC deaminase structural gene (acdS) from Rhizobium leguminosarum bv. viciae 128C53K, whereas the other 10 strains are 83% identical compared to the acdS of R. leguminosarum bv. viciae 128C53K. Southern hybridization showed that each strain has only one ACC deaminase gene. Using inverse PCR, the region upstream of the ACC deaminase structural genes was characterized for all 17 strains and shown to encode a leucine responsive regulatory protein. The results are discussed in the context of a previously proposed model for the regulation of bacterial ACC deaminase and facilitates an elaboration of the role of ACC deaminase in nodulation and nitrogen fixation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".