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Elf3 plays a role in regulating dendritic cell maturation and function (100.42)

2011· article· en· W855019242 on OpenAlexaff
Rahul Kushwah, Jing Wu, Jordan Oliver, Jim Hu

Bibliographic record

VenueThe Journal of Immunology · 2011
Typearticle
Languageen
FieldImmunology and Microbiology
TopicReproductive System and Pregnancy
Canadian institutionsHospital for Sick ChildrenUniversity of Toronto
Fundersnot available
KeywordsCell biologyDendritic cellOvalbuminImmunologyDownregulation and upregulationTranscription factorBiologyInflammationCytokineImmune systemChemistryGeneBiochemistry

Abstract

fetched live from OpenAlex

Abstract Elf3 belongs to the family of epithelium-specific Ets transcription factors and can be upregulated in non-epithelial cells upon cytokine stimulation. Among Ets family transcription factors, Elf3 is most highly induced during inflammation, yet its function is not well understood. Here we demonstrate an important role of Elf3 in regulating dendritic cell (DC) function. Intraperitoneal injection of ovalbumin (OVA)-Alum to Elf3-/- mice resulted in increased migration of DCs to the peritoneal space along with hypermaturation followed by increase in subsequent migration of DCs to the draining lymph nodes. In vitro cultured Elf3-/- DCs underwent spontaneous maturation in absence of any inflammatory stimuli and upon exposure to inflammatory stimuli underwent hypermaturation compared to Elf3+/+ DCs, indicating a role of Elf3 in regulation DC maturation. Elf3-/- mice had normal T cell development but mounted an exaggerated Th2 response upon OVA exposure. Naïve T cells cultured with Elf3-/- DCs showed impairment in Th1 and Th17 differentiation with an increased propensity for Th2 differentiation. This was mediated by an impairment of IL-6 and IL-12 production by Elf3-/- DCs. The impairment of IL-6 and IL-12 production was due to impairment of IL-6 and IL-12p40 gene induction in Elf3-/- DCs in response to inflammatory stimuli. Impairment of Th1 differentiation promoted Th2 differentiation. Taken together, our findings identify a role of Elf3 in regulating DC maturation and function.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.205
Teacher spread0.191 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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