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Elf3 regulates dendritic cell driven T cell differentiation (99.1)

2010· article· en· W86154957 on OpenAlexaff
Rahul Kushwah, Jing Wu, Jordan Oliver, Jim Hu

Bibliographic record

VenueThe Journal of Immunology · 2010
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmunotherapy and Immune Responses
Canadian institutionsHospital for Sick ChildrenUniversity of Toronto
Fundersnot available
KeywordsOvalbuminCell biologyDendritic cellImmunologyBiologySensitizationT cellChemokineImmune systemRAR-related orphan receptor gammaFOXP3

Abstract

fetched live from OpenAlex

Abstract Elf3 belongs to the family of epithelium specific Ets transcription factors and can be upregulated in non-epithelial cells upon cytokine stimulation. Elf3-/- mice have normal T cell development and T cell differentiation into different T helper lineages. Here we show that upon epicutaneous sensitization with ovalbumin(OVA) followed by OVA intranasal challenge, Elf3-/- mice display a defect in Th17 induction, though OVA specific IgE and IgG1 antibody titers are several folds higher and airway inflammation is more extensive compared to Elf3+/+ mice, indicating an exaggerated Th2 response. Furthermore, intraperitoneal sensitization of Elf3-/- mice with OVA followed by OVA intranasal challenge, confirmed that Elf3-/- mice mounted an exaggerated Th2 response. Since, dendritic cells (DCs) are key players in initiation of adaptive immune response, we analyzed DC migration upon OVA challenge and showed that pulmonary DCs underwent hyperactivation in Elf3-/- mice compared to Elf3+/+ mice and rates of DC migration to the airway draining lymph nodes were also significantly higher in Elf3-/- mice, indicating that DCs may mediate the exaggerated Th2 response observed in Elf3-/- mice. Further analysis revealed that Elf3-/- DCs are more prone to maturation than Elf3+/+ DCs and upon maturation, Elf3-/- DCs preferentially secrete chemokines/cytokines that are important in polarizing Th2 differentiation. Taken together, these findings reveal a role of Elf3 in DC driven T cell differentiation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.208
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes1
Has abstractyes

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