Role of Rho‐mediated processes and intact actin cytoskeleton in leptin induced cardiomyocytes hypertrophy
Bibliographic record
Abstract
Obesity is associated with increased leptin production which may contribute to cardiac hypertrophy. Although leptin has been shown to produce cardiomyocyte hypertrophy, its mechanism of action is unknown. Activation of Rho A has been shown to be associated with cardiac pathology and we therefore determined the role of Rho/ROCK as mediators of leptin induced cell hypertrophy in cultured neonatal rat myocytes. Leptin (3.1 nM) significantly increased cell size by 32 ± 5% and leucine incorporation by 42 ± 7%. These effects were associated with significant activation of Rho A (254 ± 25%) which was significantly attenuated by the coadministration of an anti‐leptin antibody (166 ng/ml) to 70 ± 20% of control values. Both the Rho A inhibitor C3 exoenzyme and ROCK inhibitor Y27632, potently attenuated leptin‐induced increased cell size and leucine incorporation. The hypertrophic effect of leptin was associated with a 190 ± 20% increase in phosphorylation of cofilin, an actin binding protein. In addition, the increase in polymerization of actin resulted in a decreased G/F actin ratio which was significantly inhibited by both the anti‐leptin antibody and Y‐27632. Leptin‐induced hypertrophy was also attenuated by disruption of actin filaments with 50 nM latrunculin B. Our results indicate that the activation of Rho and actin dynamics plays an important role in the leptin signaling leading to the development of cell hypertrophy. This study is supported by HSFO.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".