The Anaphase Promoting Complex is Vital for Histone Metabolism in S. cerevisiae
Bibliographic record
Abstract
Proper chromosome segregation during mitosis is essential for genomic stability. The precursor required for chromosome packaging is chromatin, a repeating unit of 147bp DNA wrapped around the nucleosome core particle, which is a complex of eight histones, two each of H2A, H2B, H3 and H4. Chromatin assembly, the placement of histones onto naked DNA, depends upon the recruitment of acetylated histones to newly synthesized or damaged DNA. Research suggests a ubiquitin‐protein ligase, the Anaphase Promoting Complex (APC), is involved with both recruitment and acetylation of histones. The APC is an essential evolutionarily conserved factor required for mitotic passage. A multisubunit E3 ubiquitin ligase, the APC plays a major role in regulating the cell cycle by targeting proteins that block mitosis for proteasomal degradation. Newly acetylated histones are deposited onto DNA by the chromatin assembly factor CAF‐I. Recent studies in our lab indicate that the APC and CAF‐I cooperate for efficient mitotic chromatin assembly. The APC may also play a role in recruiting histone acetyltransferases (HATs) to the core histones. Preliminary experiments indicate that various HAT single mutant phenotypes are exacerbated by the temperature sensitive apc5 CA mutant. Furthermore we show that histone acetylation decreases in APC mutants, as does APC substrate degradation. We propose that cell proliferation is promoted by HAT activity in an APC‐dependent manner. As unregulated chromatin formation has been linked to a variety of diseases, including cancer, understanding these mechanisms are key to understanding disease onset and progression. This work is funded by the CIHR.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".