Expression of the Sodium‐neutral amino acid exchanger ASCT2 along the small intestinal crypt‐villus axis in the formula‐fed neonatal pig
Bibliographic record
Abstract
The sodium‐neutral amino acid (AA) exchanger ASCT2 gene expressesATB0 protein responsible for the exchange of gut luminal neutral AAacross the apical membrane. The study was to quantify the ASCT2mRNA and protein expression along the jejunal crypt‐villus axis with six Yorkshiregilts at 14–16 d of age fed a milk‐based liquid formula. Threeepithelial cell fractions, representing cells from the uppervillus, the middle villus and the crypt regions, were sequentiallyisolated, with cell viability of 92–95% as assessed bytrypan blue exclusion, along the crypt‐villus axis from theentire jejunum by the distended sac method. Real time RT‐PCR analyses (SmartCycler using SYBRGreen‐I detection kit) revealed a linear decrease (P<0.05)in the ASCT2 mRNA level from the crypt cell to the upper villus cell with β‐actin as thehouse‐keeping control. No significant differences ( P >0.05) were observed in ASCT2 protein abundance in cellular homogenate, soluble, and brush border membrane among the cell fractions. These results suggest that neonatal pig enterocytes express ATB0 in the small intestinal enterocyte during the entire process of the cell proliferation and differentiation and the expression of ATB0 is not only regulated at transcriptional level but also at the post transcriptional levels. Supported by NSERC Discovery Program.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".