Phosphatidylinositol 4‐phosphate dynamics during phagocytosis
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Phosphoinositide levels undergo acute changes during the course of phagocytosis; phosphatidylinositol 4,5‐ bis phosphate [PtdIns(4,5)P 2 ], phosphatidylinositol 3,4,5‐ tris phosphate and phosphatidylinositol 3‐phosphate (PtdIns3P) undergo marked changes. However, the fate of phosphatidylinositol 4‐phosphate (PtdIns4P) has not been investigated. Recently, a genetically encoded biosensor was developed to visualize PtdIns4P in live cells. The probe, called P4M2x, consists of two copies of the PtdIns4P‐binding domain of SidM, a Legionella pneumophila effector , tagged with a fluorescent protein. Expression of P4M2x in macrophages revealed tri‐phasic changes of PtdIns4P during phagocytosis. PtdIns4P, normally present in the plasma membrane, underwent a transient, moderate increase as the phagosome sealed, coinciding with the disappearance of PtdIns(4,5)P 2 . This initial increase was followed by the virtual disappearance of PtdIns4P, which coincided with accumulation of PtdIns3P. At the time when PtdIns3P disappeared, a gradual reacquisition of PtdIns4P was observed, exceeding the levels normally found at the plasma membrane. This reappearance of PtdIns4P was preceded by the sequential acquisition of Rab5 followed by Rab7 –early and late maturation indicators, respectively. The reacquisition of PtdIns4P was accompanied by the appearance of P4M2x‐positive tubules that extended dynamically between phagosomes and the pericentriolar Golgi area.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it