Phosphatidylinositol 4‐phosphate dynamics during phagocytosis
Bibliographic record
Abstract
Phosphoinositide levels undergo acute changes during the course of phagocytosis; phosphatidylinositol 4,5‐ bis phosphate [PtdIns(4,5)P 2 ], phosphatidylinositol 3,4,5‐ tris phosphate and phosphatidylinositol 3‐phosphate (PtdIns3P) undergo marked changes. However, the fate of phosphatidylinositol 4‐phosphate (PtdIns4P) has not been investigated. Recently, a genetically encoded biosensor was developed to visualize PtdIns4P in live cells. The probe, called P4M2x, consists of two copies of the PtdIns4P‐binding domain of SidM, a Legionella pneumophila effector , tagged with a fluorescent protein. Expression of P4M2x in macrophages revealed tri‐phasic changes of PtdIns4P during phagocytosis. PtdIns4P, normally present in the plasma membrane, underwent a transient, moderate increase as the phagosome sealed, coinciding with the disappearance of PtdIns(4,5)P 2 . This initial increase was followed by the virtual disappearance of PtdIns4P, which coincided with accumulation of PtdIns3P. At the time when PtdIns3P disappeared, a gradual reacquisition of PtdIns4P was observed, exceeding the levels normally found at the plasma membrane. This reappearance of PtdIns4P was preceded by the sequential acquisition of Rab5 followed by Rab7 –early and late maturation indicators, respectively. The reacquisition of PtdIns4P was accompanied by the appearance of P4M2x‐positive tubules that extended dynamically between phagosomes and the pericentriolar Golgi area.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".