Bibliographic record
Abstract
β-1,3-葡聚糖酶是一种植物病程相关蛋白,在植物抵御病害中有重要作用。本研究以毛竹为实验材料,利用RACE技术,克隆毛竹β-1,3-葡聚糖酶基因的外显子序列,并在β-1,3-葡聚糖酶基因的编码区两端设计引物,以毛竹基因组DNA为模版扩增该基因的内含子序列。序列结果表明,该基因全长1693bp,包含两个外显子和一个内含子,命名为PheGLU(GenBank登录号GU238236)。该基因包含1个996bp的开放阅读框,编码332个氨基酸,相对分子质量为3.541×104Da,其等电点pI为6.389,是一个酸性蛋白且分泌到胞外;其中,该蛋白的二级结构中包含35.15%的α-螺旋,21.82%的β-转角,43.03%的延伸链和无规则卷曲等;三级结构同源建模预测显示,它与大麦β-1,3-葡聚糖酶(PDB number:1ghsA)具有80.4%的同源性;聚类分析显示,该基因序列与已报道的其它植物具有较的高氨基酸序列同源性。本研究为进一步鉴定毛竹β-1,3-葡聚糖酶基因的抗真菌病害能力奠定了基础。
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".