Bibliographic record
Abstract
RNA沉默是一种序列特异性的RNA降解过程，主要作用于同源性较高的转录产物，它广泛存在于各种生物中，在植物中称转录后基因沉默(Pos-Transcriptional Gene Silencing，PTGS)、动物中称为RNA干扰(RNA interference，RNAi)、真菌中被称为RNA压制(RNA quelling)。病毒诱导转录后基因沉默是由携带基因片段的病毒载体感染植物引起相应的寄主基因沉默的现象，是一种典型的RNA沉默现象。本论文建立了病毒载体诱导转录后基因沉默系统，并运用该体系研究烟草(Nicotiana benthamiana)的1，5二磷酸核酮糖羧化酶／加氧酶小亚基(Ribulose-1，5-bisphosphate(RuBP)carboxylase／oxylases small subunit,rheS)基因的部分功能。具体包括以下内容：1、分别用携带与1，5二磷酸核酮糖羧化酶／加氧酶小亚基(r'beS)基因同源的eDNA片段的烟草脆裂病毒载体(pTV．rbcS)和马铃薯X病毒载体(pgRl07．rbcS)侵染烟草(Nicotianabenthamiana)，诱导内源rbcS基因沉默；2、分别用携带与八氢番茄红素脱氢酶(phytoenedesaturase，PDS)基因同源的cDNA片段的烟草脆裂病毒载体(pTV．PDS)和马铃薯X病毒载体(pgRl07．PDS)侵染烟草(Nieotianabenthamiana)，诱导内源PDS基因沉默；3、利用携带与绿色荧光蛋白(GFP)同源的eDNA片段的烟草脆裂病毒载体(pTV．P)侵染转基因烟草(Nicotiana benthamiana 16c)，诱导转基因烟草的GFP基因沉默。4、运用该沉默系统在以下几个水平研究rbcS基因功能：(1)、rbcS基因沉默后的表型分析；(2)、rbcS基因沉默后的转录水平分析；(3)、rbcS基因沉默后的蛋白质表达水平分析；(4)、运用HPLC方法，定量分析rbcS基因沉默后的光合色素变化；(5)、用红外气体分析仪(LI-6400P，LI—COR Inc．)测定烟草rbcS基因沉默的一些重要生理指标；(6)、用两因素方差分析模型和F检验结合分子生物学证据分析病毒载体作为研究基因功能的媒介的可行性以及运用病毒诱导
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".