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Epidermal ADAM17 provides barrier immunity and prevents myeloproliferative disease by regulating Notch-AP1 interaction (166.7)

2011· article· en· W973448500 on OpenAlexaff
Aditya Murthy, Yang Shao, Juan Guinea‐Viniegra, Swami Narala, Juan Carlos Zúñiga‐Pflücker, Erwin F. Wagner, Rama Khokha

Bibliographic record

VenueThe Journal of Immunology · 2011
Typearticle
Languageen
FieldMedicine
TopicMonoclonal and Polyclonal Antibodies Research
Canadian institutionsSunnybrook Health Science CentreSunnybrook HospitalUniversity of TorontoOntario Institute for Cancer Research
Fundersnot available
KeywordsNotch signaling pathwayInflammationCell biologyImmunologyImmune systemBiologyAP-1 transcription factorCytokineSignal transductionTranscription factor

Abstract

fetched live from OpenAlex

Abstract Epithelial cells are the major component of mucosal tissues such as the skin, gut and lungs. They are critical barriers against environmental stress factors which induce significant immunopathology. Keratinocytes in the skin may act as key decision-makers of immune cell function by directing local adaptive immune responses and hematopoietic development, however mechanisms driving epithelial:immune crosstalk are incompletely understood. The cell surface sheddase A Disintegrin and Metalloproteinase 17 (ADAM17) cleaves numerous proteins with key roles in development, stress response and inflammation. Here we show that loss of epidermal Adam17 leads to severe atopic dermatitis and myeloproliferative disease. ADAM17 deficiency in keratinocytes enhanced AP1 signaling, increasing the production of epithelial cytokines (e.g. TSLP, IL-33) to drive Th2-polarized lymphocyte activation. We also observed the release of myeloid growth factors (e.g. G-CSF), causal to myeloproliferative disease in these mice. Next, we identified that ADAM17 promotes ligand-independent Notch activation in keratinocytes, revealing a novel mechanism of Notch-AP1 interaction that abrogates stress signaling. Notably, ectopic Notch activation in the epidermis of ADAM17 deficient mice rescued local inflammation and myeloproliferation. Thus Notch activation by ADAM17 in keratinocytes antagonizes AP1-induced inflammation, thereby establishing a mechanism of epithelial:immune crosstalk.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.039
GPT teacher head0.313
Teacher spread0.274 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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