The Na+‐neutral amino acid transporter ASCT2 gene is down‐regulated along the jejunal crypt‐villus axis quantified by real‐time RT‐PCR in formula‐fed neonatal pigs
Bibliographic record
Abstract
The Na+‐neutral amino acid (AA) transporter ASCT2 gene expresses ATB0 protein responsible for the uptake of luminal neutral AA across the apical membrane. The study was to quantify the ASCT2 mRNA expression along the jejunal crypt‐villus axis in six Yorkshire gilts at 14‐16 d of age fed a milk‐based liquid formula. Three epithelial cell fractions, representing cells from the upper villus, the middle villus and the crypt regions, were sequentially isolated, with cell viability of 92–95% as assessed by trypan blue exclusion, along the crypt‐villus axis from the entire jejunum by the distended sac method. The 526‐bp partial mRNA sequence of the porcine neutral AA transporter ASCT2 gene, obtained according to the conserved region of the human gene, showed 90 and 84% homology with the human and mouse sequences, respectively. Real time RT‐PCR analyses (SmartCycler using SYBR Green‐I detection kit) revealed a linear decrease (P<0.05) in the ASCT2 mRNA level from the crypt cell, to the middle villus cell and the upper villus cell with β‐actin as the house‐keeping control. These results suggest that ASCT2 gene expression is down‐regulated from the small intestinal crypt to the villus cells at in the formula‐fed neonatal pig.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".