Bibliographic record
Abstract
植物中的苹果酸酶能够参与光合作用、呼吸作用和脂类生成等多种重要的代谢途径,而且与植物的逆境防御有着密切的关系。为研究水稻中一个苹果酸酶基因（OsNAD-ME1,GeneID：4343294）的基本特征及其与逆境间的响应关系,克隆得到该基因的cDNA片段,应用生物信息学对其序列进行分析及预测,并运用Northern检测的方法,对该基因在多种非生物逆境下的表达情况做出研究。由序列分析结果可知,OsNAD-ME1的ORF为1869bp,编码622个氨基酸,OsNAD-ME1属于植物NAD-ME的α亚组,其理论分子量为68.9kD,理论等电点为6.03。预测结果表明该蛋白属于苹果酸酶家族,无跨膜结构域,定位于线粒体上。并利用GFP定位的方法,在拟南芥悬浮细胞中对亚细胞定位的预测结果进行了验证。Northern检测结果显示,OsNAD-ME1能够响应多种非生物逆境,在NaCl和NaHCO3胁迫下其表达水平在24h时达到最大,在PEG和H2O2胁迫下其表达水平在12h时达到最大。该结果表明OsNAD-ME1可能与植物对非生物胁迫的响应有关。
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.002 | 0.003 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".