Assessment of promoter function and enhancing disease resistance in transgenic carrot (Daucus carota L.)
Notice bibliographique
Résumé
Genetic engineering is a promising strategy for creating agriculturally significant resistance in carrot. Several different transgenic strategies, in addition to conducting a detailed quantification of commonly used promoters to achieve disease resistance were investigated. Tissue-specific patterns and levels of protein expression were characterized in transgenic carrot plants transformed with the ?-glucuronidase gene driven by five promoters: Cauliflower moasaic virus 35S, double 35S (D35S), Arabidopsis ubiquitin (UBQ3), mannopine synthase (mas2) or rooting loci promoter (rolD). UBQ3 promoter provided the highest levels of expression in roots, while D35S and 35S promoters had high expression in leaves. rolD and Mas2 promoters had enhanced root expression; however levels were much lower compared to the constitutive promoters, which were subsequently used in this research. Genes encoding a rice peroxidase (POC1), wheat chitinase (383) and ?1,3- glucanase (638) were introduced into carrot. Lines expressing 638 alone had no enhanced resistance to B. cinerea or S. scleorotiorum, while 383 lines reduced disease symptoms by up to 40%. When 638 and 383 were co-expressed resistance levels were similar to 383 alone. High levels of disease resistance were seen in lines expressing POC1 with 70-90% reduction in symptoms to B. cinerea and S. sclerotiorum. POC1 lines had 20-30% increases in lignin levels in petioles and roots, which was enhanced with pathogen challenge. Several defence genes exhibited strong induction in POC1 expressing plants when induced with fungal cell wall elicitor. Additionally, POC1 lines had reduced H2O2 accumulation during oxidative burst response. POC1 derived resistance was effective towards necrotrophic pathogens. Over-expression of Arabidopsis Nonexpressor of Pathogenesis Related protein 1 was studied in carrot, with two independent lines successfully generated and analyzed. There was no detectable activation of the systemic acquire resistance (SAR) pathways in the absence of pathogens, however, the lines exhibited more intense and longer lasting activation of SAR when elicited with fungal cell walls or Salicylic acid. Both lines were highly resistant against biotrophic and necrotrophic foliar pathogens and the roots were resistant towards A. radicina, indicating broad-spectrum disease resistance. The results from this study demonstrate the feasibility of engineering disease resistance in carrot using several different approaches.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,002 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».