Oxidative Stress on Canine Semen Before and after Oral Antioxidant Supplementation.
Notice bibliographique
Résumé
The spermatozoon, constantly faces the oxygen paradox: oxygen is required for life, but oxidative metabolism of biological molecules can be potentially toxic because of the formation of highly reactive oxygen species (ROS) that can modify cell viability, causing sub or infertility. The concentration of ROS in seminal plasma is inversely related to sperm quality. The imbalance between ROS antioxidant concentration cause oxidative stress on semen. Oxidative stress has been proposed to have a causal/concausal role in many cases of male infertility. So, many treatments with antioxidants has been proposal to decrease the problems cause by oxidative stress. In humans, oral administration of vitamin E improved sperm function and gave a significant dose-dependent suppression of ROS production from human spermatozoa. Ascorbate is most effective as an antioxidant in the aqueous phase, and its role might be to scavenge free radicals and regenerate Vitamin E. The aim of this work was to evaluate the oxidative stress on seminal plasma from subfertile dogs after oral supplementation with vitamin C and E. Six healthy and sexually mature dogs were used in this study: four Labrador Retrievers, one Rottweiller and one German Sheperd, ranging between 2 and 7 years of age. Those dogs had low sperm count (<20x106sptz/mL) and/or more than 30% of total sperm pathology. They were all from Military Police from Bauru-SP- Brazil and were fed with a commercial dog food (Maxi adulto for large breeds; Royal Canin,Brazil).The dogs had their diet supplemented with vitamin C and E (500 µg each)for 60 days. Semen was collected, from those dogs, by digital manipulation. The ejaculate was diluted with TRIS/egg-yolk extender and then centrifuged twice, in order to obtain seminal plasma without sperm cells and remove as much as possible the extender. The concentration of thiobarbituric acid-reactive substances (TBARS) were measured in seminal plasma before, after 30 and 60 days of supplementation. The TBARS concentration were used as index of oxidative stress. Results were analyzed using repeated measures ANOVA. No statistic difference were found on TBARS concentration, between the 3 moments evaluated. Based on these findings, it can be concluded that the oral supplementation for 60 days, with 500 µg of vitamin C and E does not decrease the oxidative stress on canine seminal plasma from subfertile dogs. Acknowledges: FAPESP, Royal Canin, Poilicia Militar de Bauru. (poster)
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».