Oxidative Stress on Canine Semen Before and after Oral Antioxidant Supplementation.
Bibliographic record
Abstract
The spermatozoon, constantly faces the oxygen paradox: oxygen is required for life, but oxidative metabolism of biological molecules can be potentially toxic because of the formation of highly reactive oxygen species (ROS) that can modify cell viability, causing sub or infertility. The concentration of ROS in seminal plasma is inversely related to sperm quality. The imbalance between ROS antioxidant concentration cause oxidative stress on semen. Oxidative stress has been proposed to have a causal/concausal role in many cases of male infertility. So, many treatments with antioxidants has been proposal to decrease the problems cause by oxidative stress. In humans, oral administration of vitamin E improved sperm function and gave a significant dose-dependent suppression of ROS production from human spermatozoa. Ascorbate is most effective as an antioxidant in the aqueous phase, and its role might be to scavenge free radicals and regenerate Vitamin E. The aim of this work was to evaluate the oxidative stress on seminal plasma from subfertile dogs after oral supplementation with vitamin C and E. Six healthy and sexually mature dogs were used in this study: four Labrador Retrievers, one Rottweiller and one German Sheperd, ranging between 2 and 7 years of age. Those dogs had low sperm count (<20x106sptz/mL) and/or more than 30% of total sperm pathology. They were all from Military Police from Bauru-SP- Brazil and were fed with a commercial dog food (Maxi adulto for large breeds; Royal Canin,Brazil).The dogs had their diet supplemented with vitamin C and E (500 µg each)for 60 days. Semen was collected, from those dogs, by digital manipulation. The ejaculate was diluted with TRIS/egg-yolk extender and then centrifuged twice, in order to obtain seminal plasma without sperm cells and remove as much as possible the extender. The concentration of thiobarbituric acid-reactive substances (TBARS) were measured in seminal plasma before, after 30 and 60 days of supplementation. The TBARS concentration were used as index of oxidative stress. Results were analyzed using repeated measures ANOVA. No statistic difference were found on TBARS concentration, between the 3 moments evaluated. Based on these findings, it can be concluded that the oral supplementation for 60 days, with 500 µg of vitamin C and E does not decrease the oxidative stress on canine seminal plasma from subfertile dogs. Acknowledges: FAPESP, Royal Canin, Poilicia Militar de Bauru. (poster)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".