321 OVARIAN SUPERSTIMULATION AND OOCYTE COLLECTION IN WOOD BISON, A THREATENED CANADIAN SPECIES
Notice bibliographique
Résumé
The wood bison (Bison bison athabascae) is a threatened species, and recovery is constrained by endemic tuberculosis and brucellosis in wild herds in and around Wood Buffalo National Park, Alberta, Canada. The Committee on the Status of Endangered Wildlife in Canada has recommended the eradication of affected herds and repopulation with healthy bison obtained through the use of reproductive technologies. As part of the conservation effort, the specific objective of this study was to develop an effective ovarian superstimulatory protocol in bison that will permit collection of a large number of oocytes for the ultimate purpose of in vitro embryo production. Ovarian function was synchronized among female wood bison during the ovulatory season (December) by giving a luteolytic dose of prostaglandin followed 8 days later by transvaginal ultrasound-guided follicular ablation. On the day after follicular ablation (expected day of follicular wave emergence, Day 0), bison were assigned randomly to 2 groups (n = 11/group) and given either a single intramuscular dose of 2500 IU of eCG or subcutaneous doses of 200 mg of FSH given on Days 0 and 2. A luteolytic dose of prostaglandin was given on Day 2, and 25 mg of LH was given intramuscularly on Day 4. Cumulus–oocyte complexes (COC) were collected 24 h after LH treatment by transvaginal ultrasound-guided follicle aspiration using an 18 G × 2’ aspiration needle attached to a flexible tubing and a regulated vacuum pump (flow rate, 22 mL min–1). The COC were classified morphologically from 1 (excellent) to 4 (poor) based on attributes of the ooplasm and the number of surrounding cumulus cells. The number of COC in grade 1 to 3 was combined (i.e. grades acceptable for IVF) for purposes of statistical interpretation. Ovarian response and oocyte quality were compared between groups by t-tests, and COC collection rate was compared by chi-square analysis. The number (mean ± SEM) of follicles =5 mm was greater (P < 0.05) in bison treated with FSH than in those treated with eCG (12.2 ± 1.7 v. 5.8 ± 0.5), resulting in a greater number of follicles aspirated (11.2 ± 1.8 v. 5.6 ± 0.5; P < 0.05), more COC collected (7.2 ± 1.4 v. 3.4 ± 0.6; P < 0.05), and more COC of acceptable quality (6.1 ± 1.4 v. 2.5 ± 0.5). The COC collection rate (i.e. number of COC collected per number of follicles aspirated), however, did not differ between FSH- and eCG-treated groups [79/123 (64%) v. 37/62 (60%)]. The results document the successful collection of viable oocytes from superstimulated wood bison via tranvaginal ultrasound-guided follicle aspiration. The number of large follicles, follicles aspirated, and COC collected were more than twice as high in bison treated with FSH than in those treated with eCG. This work was supported by grants from Advancing Canadian Agriculture and Agri-Food Fund and Agri-Food Innovation Fund. The authors thank Bioniche Animal Health Canada for providing Folltropin, Lutropin, and Pregnecol.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».