Differential Amplification of Intron-containing Transcripts Reveals Long Term Potentiation-associated Up-regulation of Specific Pde10A Phosphodiesterase Splice Variants

We employed differential display of expressed mRNAs (Liang, P., and Pardee, A. B. (1992) Science 257, 967–971) to identify genes up-regulated after long term potentiation (LTP) induction in the hippocampus of awake adult rats. In situ hybridization confirmed the differential expression of five independently amplified clones representing two distinct transcripts, cl13/19/90 and cl95/96. Neither cl13/19/90 nor cl95/96 showed significant sequence homology to known transcripts (mRNA or expressed sequence tag) or to the mouse or human genome. However, comparison with the rat genome revealed that they are localized to of the cl13/19/90 and cl95/96 are to of the distinct of the and in situ hybridization that of are up-regulated after that of and that differential display to identify differential expression of In the in of transcripts of the in long term We employed differential display of expressed mRNAs (Liang, P., and Pardee, A. B. (1992) Science 257, 967–971) to identify genes up-regulated after long term potentiation (LTP) induction in the hippocampus of awake adult rats. In situ hybridization confirmed the differential expression of five independently amplified clones representing two distinct transcripts, cl13/19/90 and cl95/96. Neither cl13/19/90 nor cl95/96 showed significant sequence homology to known transcripts (mRNA or expressed sequence tag) or to the mouse or human genome. However, comparison with the rat genome revealed that they are localized to of the cl13/19/90 and cl95/96 are to of the distinct of the and in situ hybridization that of are up-regulated after that of and that differential display to identify differential expression of In the in of transcripts of the in long term the of in the in that in are of in the term potentiation (LTP) long term of long term long term of long term long in in the hippocampus and and the and that of and and the of in the induction of are in and A. B. A. B. B. that and and in the in the of the and the of long term and the of B. that and to genes that are transcripts and A. with A. B. A. A. A. B. In to and with the induction of A. B. A. of the to B. in expression or of transcripts the expression of A. B. A. A. A. A. expression of transcripts and to with and the of long term A. B. A. A. A. and are to the of the long term and employed differential display of expressed mRNAs to identify genes in the awake differential display to identify in expression of the of to identify differential expression of in situ to the differential expression of five clones that two transcripts, cl13/19/90 and cl95/96. We clones are of the to of of A. of the in long term and in the in the induction and of in the awake rat A. B. in with the of the and the display A. B. that to the differential of cl13/19/90 and cl95/96 and and and and and and in to to to to or after to and to in situ and In situ hybridization B. in in and and in in of long and in and in to to the of and and to the to or in expressed of the expression of the in expression with the in and of or with adult rat and to the of with of of in the of of of and in of in of the of the to of the and and and and and and and and and and and and and to of and and to and to and and rat and with the or to and the of that expressed with In of in and or to the expressed to the of in to the the are of five in two are of of of the in of the in the of that of display in and after or after and the of the to the the of the of We differential display the to transcripts transcripts to independently in situ In situ hybridization to the sequence of confirmed differential expression of five and clones that they two transcripts cl13/19/90 and cl95/96. clones up-regulated after the induction of and to in the of or in the in the of the two transcripts in situ In the adult cl95/96 and cl13/19/90 are expressed in the with expression the the of the and In to cl13/19/90 and cl95/96 are expressed in situ hybridization the to of cl13/19/90 and cl95/96 to with of B. expression and cl95/96 of the differential display to the of the of cl13/19/90 and cl95/96. and cl95/96 to in of the they to identify cl13/19/90 or cl95/96 mRNAs the of the of cl13/19/90 or cl95/96 We the sequence of cl13/19/90 and cl95/96 to the sequence significant homology to the that the of expressed sequence or the of the human or mouse genome. of homology of cl13/19/90 and cl95/96 to the of sequence of cl13/19/90 and cl95/96 homology with the genome sequence of the the differential display cl13/19/90 and cl95/96 in to Neither they the genome. confirmed transcripts are in clones are the of the of the of the of cl13/19/90 and cl95/96 to clones of the in they are In cl13/19/90 and cl95/96 of the of or However, cl13/19/90 and cl95/96 are of the to of the rat are to of of rat to and are in and are in the in situ hybridization in and of to the of cl13/19/90 and cl95/96 are of the to of the We of the in adult rat hippocampus and confirmed the of sequence are in with the of the and amplified that to the that cl13/19/90 and cl95/96. the of of they and in adult rat of of the cl13/19/90 and cl95/96 or are of the that cl13/19/90 and cl95/96 are of the of the amplified of We to in the the of the the of of adult rat cl13/19/90 and cl95/96 are of the the expression and We in situ hybridization the of rat in and the to in situ hybridization of in of and in situ of and and in situ two of of the of the of the they are and they are and and in and to the of in two distinct of and the and the to and two distinct of and the and to the the of in the the sequence of of the sequence in and and in in in situ hybridization confirmed the of and are up-regulated induction and in and are and expression in the to the of in the hippocampus or in in the of in to expression of and after the induction of to A. and to identify genes A. B. A. in situ hybridization confirmed that cl13/19/90 and cl95/96 and and are up-regulated of situ hybridization and are up-regulated the induction of and and and are and to that expression of and the of transcripts, the expression of to that of cl13/19/90 and the to that are up-regulated expression with cl13/19/90 and and expression of are of cl13/19/90 and cl95/96 the that cl13/19/90 and cl95/96 are of the cl13/19/90 and cl95/96 are of the that in situ hybridization sequence of the of expression cl13/19/90 and cl95/96. In situ hybridization sequence the of in showed and of expression to cl13/19/90 and expression that and cl13/19/90 and cl95/96 expression the that cl13/19/90 and cl95/96 situ hybridization sequence with cl13/19/90 and of expression the expression of to that of the that cl13/19/90 and cl95/96 are of the and of of the and of the the of of comparison to the In the transcripts that sequence to the and they the of adult rat and cl13/19/90 and two distinct of and the and the to two distinct of and the and to the the sequence the revealed and are or In of of the of the of the of in the of to of and and they of to sequence in the of with with sequence of of the that transcripts that the of and are the and that the of and induction in situ the of the in to the of expression and rat and expressed to the of in in expression of of with with that with in and of of In to with in and of of that and A. of expression rat or in expression of the or nor with or are in in in the to the of of of the in and However, to with in with in with with expression of the or nor with in in and with or and employed differential display of expressed mRNAs to identify genes in the hippocampus of awake rats. genes that to in situ We confirmed the differential expression of five clones representing two transcripts, cl13/19/90 and cl13/19/90 nor cl95/96 showed significant sequence homology to known (mRNA or expressed sequence tag) in or human or to sequence in or two clones sequence to two in the localized to the of the that cl13/19/90 and cl95/96 are of the of distinct the of the and in situ and are up-regulated the induction of to and and and that two of the and cl13/19/90 and cl95/96 expression to the of expression of cl13/19/90 and cl95/96 are that of the of the and of the and expression cl13/19/90 and cl95/96 that they are of cl13/19/90 or cl95/96 to identify cl13/19/90 and cl95/96 and cl13/19/90 and cl95/96 are the of differential display to identify in expression of differential display in to to that the of the of the of long to in they of and the of to B. A. A. that the in cl13/19/90 and cl95/96 are to in that of the to the of that cl13/19/90 and cl95/96 are of the the that they are of in the to and in the of sequence A. that and that of However, A. that cl13/19/90 and cl95/96 are of that in of the expression of cl13/19/90 and cl95/96 are of the of the and the clones the that the to to are the that the of expressed and to and and and to the are to the and and and of the with the sequence the of the are to of the genes to with and A. the to A. the that that in in are to to the of We that nor that the in to the that A. and that in with expression of of to in of the of of and of are expressed in the hippocampus A. A. of the in of or the induction of A. with potentiation and of in adult and of long potentiation that of the of that the up-regulated the induction of to of that with in and to that to of of of the the of the of the to and the of the of to that the the of the to and to they the the and the that are in the that of are up-regulated the induction of and are that are that of in and the of and induction in situ the of differential display of expressed two transcripts, cl13/19/90 and that are up-regulated the induction of We that clones are of the that of the to the of that differential to identify in expression of to identify differential expression of and that A. of the in long term the of in the in that in are of in the term potentiation (LTP) long term of long term long term of long term long in in the hippocampus and and the and that of and and the of in the induction of are in and A. B. A. B. B. that and and in the in the of the and the of long term and the of B. that and to genes that are transcripts and A. with A. B. A. A. A. B. In to and with the induction of A. B. A. of the to B. in expression or of transcripts the expression of A. B. A. A. A. A. expression of transcripts and to with and the of long term A. B. A. A. A. and are to the of the long term and In employed differential display of expressed mRNAs to identify genes in the awake differential display to identify in expression of the of to identify differential expression of in situ to the differential expression of five clones that two transcripts, cl13/19/90 and cl95/96. We clones are of the to of of A. of the in long term and in the in the induction and of in the awake rat A. B. in with the of the and the display A. B. that to the differential of cl13/19/90 and cl95/96 and and and and and and in to to to to or after to and to in situ and In situ hybridization B. in in and and in in of long and in and in to to the of and and to the to or in expressed of the expression of the in expression with the in and of or with adult rat and to the of with of of in the of of of and in of in of the of the to of the and and and and and and and and and and and and and to of and and to and to and and rat and with the or to and the of that expressed with In of in and or to the expressed to the of in to the the are of five in two are of In and in the in the induction and of in the awake rat A. B. in with the of the and the display A. B. that to the differential of cl13/19/90 and cl95/96 and and and and and and in to to to to or after to and to in situ In and In situ hybridization B. in in and and in in of long and in and in to to the of and and to the to or in expressed of the expression of the in expression with the in and of or with adult rat and to the of with of of in the of of of and in of in of the of the to of the and and and and and and and and and and and and and to of and and to and to and and rat and with the or to and the of that expressed with In of in and or to the expressed to the of in to the the are of five in two are of of of the in of the in the of that of display in and after or after and the of the to the the of the of We differential display the to transcripts transcripts to independently in situ In situ hybridization to the sequence of confirmed differential expression of five and clones that they two transcripts cl13/19/90 and cl95/96. clones up-regulated after the induction of and to in the of or in the in the of the two transcripts in situ In the adult cl95/96 and cl13/19/90 are expressed in the with expression the the of the and In to cl13/19/90 and cl95/96 are expressed in situ hybridization the to of cl13/19/90 and cl95/96 to with of B. expression and cl95/96 of the differential display to the of the of cl13/19/90 and cl95/96. and cl95/96 to in of the they to identify cl13/19/90 or cl95/96 mRNAs the of the of cl13/19/90 or cl95/96 We the sequence of cl13/19/90 and cl95/96 to the sequence significant homology to the that the of expressed sequence or the of the human or mouse genome. of homology of cl13/19/90 and cl95/96 to the of sequence of cl13/19/90 and cl95/96 homology with the genome sequence of the the differential display cl13/19/90 and cl95/96 in to Neither they the genome. confirmed transcripts are in clones are the of the of the of the of cl13/19/90 and cl95/96 to clones of the in they are In cl13/19/90 and cl95/96 of the of or However, cl13/19/90 and cl95/96 are of the to cl13/19/90 and cl95/96 are of the to of the We of the in adult rat hippocampus and confirmed the of sequence are in with the of the and amplified that to the that cl13/19/90 and cl95/96. the of of they and in adult rat of of the cl13/19/90 and cl95/96 or are of the that cl13/19/90 and cl95/96 are of the of the amplified of We to in the the of the the of of adult rat cl13/19/90 and cl95/96 are of the the expression and We in situ hybridization the of rat in and the to in situ hybridization of in of and in situ of and and in situ two of of the of the of the they are and they are and and in and to the of in two distinct of and the and the to and two distinct of and the and to the the of in the the sequence of of the sequence in and and in in in situ hybridization confirmed the of and are up-regulated induction and in and are and expression in the to the of in the hippocampus or in in the of in to expression of and after the induction of to A. and to identify genes A. B. A. in situ hybridization confirmed that cl13/19/90 and cl95/96 and and are up-regulated of situ hybridization and are up-regulated the induction of and and and are and to that expression of and the of transcripts, the expression of to that of cl13/19/90 and the to that are up-regulated expression with cl13/19/90 and and expression of are of cl13/19/90 and cl95/96 the that cl13/19/90 and cl95/96 are of the cl13/19/90 and cl95/96 are of the that in situ hybridization sequence of the of expression cl13/19/90 and cl95/96. In situ hybridization sequence the of in showed and of expression to cl13/19/90 and expression that and cl13/19/90 and cl95/96 expression the that cl13/19/90 and cl95/96 situ hybridization sequence with cl13/19/90 and of expression the expression of to that of the that cl13/19/90 and cl95/96 are of the and of of the and of the the of of comparison to the In the transcripts that sequence to the and they the of adult rat and cl13/19/90 and two distinct of and the and the to two distinct of and the and to the the sequence the revealed and are or In of of the of the of the of in the of to of and and they of to sequence in the of with with sequence of of the that transcripts that the of and are the and that the of and induction in situ the of the in to the of expression and rat and expressed to the of in in expression of of with with that with in and of of In to with in and of of that and A. of expression rat or in expression of the or nor with or are in in in the to the of of of the in and However, to with in with in with with expression of the or nor with in in and with or and of of the in of the in the of that of display in and after or after and the of the to the the of the of We differential display the to transcripts transcripts to independently in situ In situ hybridization to the sequence of confirmed differential expression of five and clones that they two transcripts cl13/19/90 and cl95/96. clones up-regulated after the induction of and to in the of or in the in the of the two transcripts in situ In the adult cl95/96 and cl13/19/90 are expressed in the with expression the the of the and In to cl13/19/90 and cl95/96 are expressed in situ hybridization the to of cl13/19/90 and cl95/96 to with of B. expression cl13/19/90 and cl95/96 of the differential display to the of the of cl13/19/90 and cl95/96. and cl95/96 to in of the they to identify cl13/19/90 or cl95/96 mRNAs the of the of cl13/19/90 or cl95/96 We the sequence of cl13/19/90 and cl95/96 to the sequence significant homology to the that the of expressed sequence or the of the human or mouse genome. of homology of cl13/19/90 and cl95/96 to the of sequence of cl13/19/90 and cl95/96 homology with the genome sequence of the the differential display cl13/19/90 and cl95/96 in to Neither they the genome. confirmed transcripts are in clones are the of the of the of the of cl13/19/90 and cl95/96 to clones of the in they are In cl13/19/90 and cl95/96 of the of or However, cl13/19/90 and cl95/96 are of the to We cl13/19/90 and cl95/96 are of the to of the We of the in adult rat hippocampus and confirmed the of sequence are in with the of the and amplified that to the that cl13/19/90 and cl95/96. the of of they and in adult rat of of the cl13/19/90 and cl95/96 or are of the that cl13/19/90 and cl95/96 are of the We cl13/19/90 and cl95/96 are of the the expression and We in situ hybridization the of rat in and the to in situ hybridization of in of and in situ of and and in situ two in situ hybridization confirmed the of and are up-regulated induction and in and are and expression in the to the of in the hippocampus or in in the of in to expression of and after the induction of to A. and to identify genes A. B. A. in situ hybridization confirmed that cl13/19/90 and cl95/96 and and are up-regulated the of transcripts, the expression of to that of cl13/19/90 and the to that are up-regulated expression with cl13/19/90 and and expression of are of cl13/19/90 and cl95/96 the that cl13/19/90 and cl95/96 are of the cl13/19/90 and cl95/96 are of the that in situ hybridization sequence of the of expression cl13/19/90 and cl95/96. In situ hybridization sequence the of in showed and of expression to cl13/19/90 and expression that and cl13/19/90 and cl95/96 expression the that cl13/19/90 and cl95/96 of of the and of the the of of comparison to the In the transcripts that sequence to the and they the of adult rat and cl13/19/90 and two distinct of and the and the to two distinct of and the and to the the sequence the revealed and are or In of of the of the of the of in the of to of and and they of to sequence in the of with with sequence of of the that transcripts that the of and are the and that the of and induction in situ the of the in to the of expression and rat and expressed to the of in in expression of of with with that with in and of of In to with in and of of that and A. of expression rat or in expression of the or nor with or are in in in the to the of employed differential display of expressed mRNAs to identify genes in the hippocampus of awake rats. genes that to in situ We confirmed the differential expression of five clones representing two transcripts, cl13/19/90 and cl13/19/90 nor cl95/96 showed significant sequence homology to known (mRNA or expressed sequence tag) in or human or to sequence in or two clones sequence to two in the localized to the of the that cl13/19/90 and cl95/96 are of the of distinct the of the and in situ and are up-regulated the induction of to and and and that two of the and cl13/19/90 and cl95/96 expression to the of expression of cl13/19/90 and cl95/96 are that of the of the and of the and expression cl13/19/90 and cl95/96 that they are of cl13/19/90 or cl95/96 to identify cl13/19/90 and cl95/96 and cl13/19/90 and cl95/96 are the of differential display to identify in expression of differential display in to to that the of the of the of long to in they of and the of to B. A. A. that the in cl13/19/90 and cl95/96 are to in that of the to the of that cl13/19/90 and cl95/96 are of the the that they are of in the to and in the of sequence A. that and that of However, A. that cl13/19/90 and cl95/96 are of that in of the expression of cl13/19/90 and cl95/96 are of the of the and the clones the that the to to are the that the of expressed and to and and and to the are to the and and and of the with the sequence the of the are to of the genes to with and A. the to A. the that that in in are to to the of We that nor that the in to the that A. and that in with expression of of to in of the of of and of are expressed in the hippocampus A. A. of the in of or the induction of A. with potentiation and of in adult and of long potentiation that of the of that the up-regulated the induction of to of that with in and to that to of of of the the of the of the to and the of the of to that the the of the to and to they the the and the that are in the that of are up-regulated the induction of and are that are that of in and the of and induction in situ the of differential display of expressed two transcripts, cl13/19/90 and that are up-regulated the induction of We that clones are of the that of the to the of that differential to identify in expression of to identify differential expression of and that A. of the in long term We employed differential display of expressed mRNAs to identify genes in the hippocampus of awake rats. genes that to in situ We confirmed the differential expression of five clones representing two transcripts, cl13/19/90 and cl95/96. Neither cl13/19/90 nor cl95/96 showed significant sequence homology to known (mRNA or expressed sequence tag) in or human or to sequence in or two clones sequence to two in the localized to the of the that cl13/19/90 and cl95/96 are of the of distinct the of the and in situ and are up-regulated the induction of to and and and that two of the and cl13/19/90 and cl95/96 expression to the of expression of cl13/19/90 and cl95/96 are that of the of the and of the and expression cl13/19/90 and cl95/96 that they are of cl13/19/90 or cl95/96 to identify cl13/19/90 and cl95/96 and cl13/19/90 and cl95/96 are the of differential display to identify in expression of differential display in to to that the of the of the of long to in they of and the of to B. A. A. that the in cl13/19/90 and cl95/96 are to in that of the to the of that cl13/19/90 and cl95/96 are of the the that they are of in the to and in the of sequence A. that and that of However, A. that cl13/19/90 and cl95/96 are of that in of the expression of cl13/19/90 and cl95/96 are of the of the and the clones the that the to to are the that the of expressed and to and and and to the are to the and and and of the with the sequence the of the are to of the genes to with and A. the to A. the that that in in are to to the of We that nor that the in to the that A. and that in with expression of of to in of the of of and of are expressed in the hippocampus A. A. of the in of or the induction of A. with potentiation and of in adult and of long potentiation that of the of that the up-regulated the induction of to of that with in and to that to of of of the the of the of the to and the of the of to that the the of the to and to they the the and the that are in the that of are up-regulated the induction of and are that are that of in and the of and induction in situ the of In differential display of expressed two transcripts, cl13/19/90 and that are up-regulated the induction of We that clones are of the that of the to the of that differential to identify in expression of to identify differential expression of and that A. of the in long term We and with

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