207 OOCYTE RECOVERY RATES AND IN VITRO BLASTOCYST PRODUCTION IN CATTLE TREATED WITH A SINGLE INJECTION OF FOLLTROPIN-V DILUTED IN A SLOW-RELEASE FORMULATION
Notice bibliographique
Résumé
We have previously shown that superstimulation of beef donors with a single intramuscular (IM) injection of Folltropin-V (Bioniche Animal Health, Belleville, Ontario, Canada) diluted in a proprietary slow-release formulation (SRF; Bioniche Animal Health) results in in vivo embryo production comparable with the traditional twice-daily IM administration over 4 days (Tribulo et al. 2010 Reprod. Fertil. Dev. 22, 367). Two experiments were designed to evaluate oocyte recovery and in vitro blastocyst production of oocyte donors superstimulated with Folltropin-V, administered in twice-daily injections or diluted in SRF and given by a single injection. In Experiment 1, crossbred beef cows (n = 11) and heifers (n = 5) were randomly allocated to 3 treatment groups in a crossover design (i.e. all animals were treated 3 times, and all treatments were represented in the 3 replicates). All donors had all follicles >8 mm in diameter ablated by ultrasound-guided follicle aspiration on Day 0, received 500 µg of cloprostenol (prostaglandin F2a, Ciclase, Syntex, Argentina), and were treated as follows: Group 1 (multiple FSH): 160 mg of Folltropin-V divided into 4 IM injections administered twice daily (i.e. Days 1 and 2); Group 2 (single FSH): 160 mg of Folltropin-V diluted in 4 mL of 25% SRF and given by a single IM injection in the neck on Day 1; Group 3 (control): no FSH treatment. On Day 4, oocytes were aspirated from all follicles >3 mm in diameter by ultrasound-guided follicular aspiration, classified, matured in TCM-199 supplemented with 0.4% BSA, fertilized in Fert medium, and in vitro cultured in SOF supplemented with 0.4% BSA under oil at 37°C, 5% CO2, 5% O2, 90% N2, and saturated humidity for 7 days. Data were analyzed by ANOVA. The mean (±SEM) numbers of follicles aspirated and oocytes recovered were higher (P < 0.05) in Groups 1 (multiple FSH, 13.6 ± 1.9 and 8.5 ± 1.0) and 2 (single FSH, 14.7 ± 1.7 and 9.4 ± 1.3) than in Group 3 (control, 8.7 ± 0.9 and 5.8 ± 1.0). The mean number of blastocysts after 7 days in culture was numerically higher (P = 0.2) in Groups 1 (2.1 ± 0.7) and 2 (2.6 ± 0.6) than in Group 3 (1.1 ± 0.4). In Experiment 2, lactating Holstein cows (n = 10) received 2.5 mg of estradiol benzoate and 50 mg of progesterone IM on Day 0. On Day 4, all cows received prostaglandin F2a and were randomly allocated to 2 treatment groups, to receive 200 mg of Folltropin-V divided into 4 twice-daily IM injections or 200 mg of Folltropin-V diluted in 25% SRF and given as a single IM injection. On Day 7, oocytes were collected and treated like those in Experiment 1. The mean (±SEM) numbers of follicles aspirated (21.2 ± 3.5 and 14.4 ± 2.9), oocytes recovered (10.2 ± 1.2 and 13.8 ± 2.9), and blastocysts produced (4.6 ± 0.7 and 6.0 ± 2.0) did not differ for Groups 1 and 2, respectively (P = 0.3). In conclusion, superstimulatory treatment increased the number of cumulus–oocyte complexes available for IVF compared with no treatment, and ovarian superstimulatory treatment may be simplified by the use of a single IM dose of a sustained-release product, compared with multiple doses of a conventional product, without a reduction in the recovery or quality of cumulus–oocyte complexes.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».