207 OOCYTE RECOVERY RATES AND IN VITRO BLASTOCYST PRODUCTION IN CATTLE TREATED WITH A SINGLE INJECTION OF FOLLTROPIN-V DILUTED IN A SLOW-RELEASE FORMULATION
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Bibliographic record
Abstract
We have previously shown that superstimulation of beef donors with a single intramuscular (IM) injection of Folltropin-V (Bioniche Animal Health, Belleville, Ontario, Canada) diluted in a proprietary slow-release formulation (SRF; Bioniche Animal Health) results in in vivo embryo production comparable with the traditional twice-daily IM administration over 4 days (Tribulo et al. 2010 Reprod. Fertil. Dev. 22, 367). Two experiments were designed to evaluate oocyte recovery and in vitro blastocyst production of oocyte donors superstimulated with Folltropin-V, administered in twice-daily injections or diluted in SRF and given by a single injection. In Experiment 1, crossbred beef cows (n = 11) and heifers (n = 5) were randomly allocated to 3 treatment groups in a crossover design (i.e. all animals were treated 3 times, and all treatments were represented in the 3 replicates). All donors had all follicles >8 mm in diameter ablated by ultrasound-guided follicle aspiration on Day 0, received 500 µg of cloprostenol (prostaglandin F2a, Ciclase, Syntex, Argentina), and were treated as follows: Group 1 (multiple FSH): 160 mg of Folltropin-V divided into 4 IM injections administered twice daily (i.e. Days 1 and 2); Group 2 (single FSH): 160 mg of Folltropin-V diluted in 4 mL of 25% SRF and given by a single IM injection in the neck on Day 1; Group 3 (control): no FSH treatment. On Day 4, oocytes were aspirated from all follicles >3 mm in diameter by ultrasound-guided follicular aspiration, classified, matured in TCM-199 supplemented with 0.4% BSA, fertilized in Fert medium, and in vitro cultured in SOF supplemented with 0.4% BSA under oil at 37°C, 5% CO2, 5% O2, 90% N2, and saturated humidity for 7 days. Data were analyzed by ANOVA. The mean (±SEM) numbers of follicles aspirated and oocytes recovered were higher (P < 0.05) in Groups 1 (multiple FSH, 13.6 ± 1.9 and 8.5 ± 1.0) and 2 (single FSH, 14.7 ± 1.7 and 9.4 ± 1.3) than in Group 3 (control, 8.7 ± 0.9 and 5.8 ± 1.0). The mean number of blastocysts after 7 days in culture was numerically higher (P = 0.2) in Groups 1 (2.1 ± 0.7) and 2 (2.6 ± 0.6) than in Group 3 (1.1 ± 0.4). In Experiment 2, lactating Holstein cows (n = 10) received 2.5 mg of estradiol benzoate and 50 mg of progesterone IM on Day 0. On Day 4, all cows received prostaglandin F2a and were randomly allocated to 2 treatment groups, to receive 200 mg of Folltropin-V divided into 4 twice-daily IM injections or 200 mg of Folltropin-V diluted in 25% SRF and given as a single IM injection. On Day 7, oocytes were collected and treated like those in Experiment 1. The mean (±SEM) numbers of follicles aspirated (21.2 ± 3.5 and 14.4 ± 2.9), oocytes recovered (10.2 ± 1.2 and 13.8 ± 2.9), and blastocysts produced (4.6 ± 0.7 and 6.0 ± 2.0) did not differ for Groups 1 and 2, respectively (P = 0.3). In conclusion, superstimulatory treatment increased the number of cumulus–oocyte complexes available for IVF compared with no treatment, and ovarian superstimulatory treatment may be simplified by the use of a single IM dose of a sustained-release product, compared with multiple doses of a conventional product, without a reduction in the recovery or quality of cumulus–oocyte complexes.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it