The Role of the ϵ Subunit in the Escherichia coli ATP Synthase

The role of the C-domain of the ϵ subunit of ATP synthase was investigated by fusing either the 20-kDa flavodoxin (Fd) or the 5-kDa chitin binding domain (CBD) to the N termini of both full-length ϵ and a truncation mutant ϵ88-stop. All mutant ϵ proteins were stable in cells and supported F1F0 assembly. Cells expressing the Fd-ϵ or Fd-ϵ88-stop mutants were unable to grow to of of The of ϵ supported Fd-ϵ of the the ATP synthase were the in and the Fd-ϵ88-stop of ATP and of ATP to the the role of the C-domain in of the and mutant of ATP to of the of ATP the the of the C-domain in the ϵ subunit to to The role of the C-domain of the ϵ subunit of ATP synthase was investigated by fusing either the 20-kDa flavodoxin (Fd) or the 5-kDa chitin binding domain (CBD) to the N termini of both full-length ϵ and a truncation mutant ϵ88-stop. All mutant ϵ proteins were stable in cells and supported F1F0 assembly. Cells expressing the Fd-ϵ or Fd-ϵ88-stop mutants were unable to grow to of of The of ϵ supported Fd-ϵ of the the ATP synthase were the in and the Fd-ϵ88-stop of ATP and of ATP to the the role of the C-domain in of the and mutant of ATP to of the of ATP the the of the C-domain in the ϵ subunit to to ATP synthase the the of ATP the of and and the in the in a to ATP the and The the of a of and the binding The a and of a of ATP the and the of the of the in both the binding of and and the and of ATP by the binding of The the a the and to ATP synthase and the to and the of ϵ the of and to the a or a The of the by both and a domain the and a domain the The or in the of the the of The of ϵ in the ATP synthase of the and the in The in the in both in the the of the C-domain and to in the the and to the of the the of ATP ϵ the and ATP by in the of The ϵ subunit was to the ATP ATP synthase to either or of in by fusing proteins of to the of ϵ to the proteins was to by binding binding of the the of the by the in a the C-domain of ϵ a in ATP the by in the ATP the of the C-domain by of the and the to ϵ in the ATP synthase by ATP and to in ATP the of the of by a of and a of the of a 20-kDa to the N of ϵ and ATP The of was the C-domain was of the C-domain of ϵ in of ATP a of the of the C-domain to ATP The the of the were by All by and were by were by the of of ATP and were by of ATP were the The was by the of of and either or was The ϵ subunit was and were by and the and ϵ the domain of ϵ by of the a the ϵ mutants was of were and to the to the by in The was a and of the of and the was by and of The ϵ the ϵ mutants in the ATP synthase were and in the of ϵ the the was the of the ϵ of the the the and or the were and and the to and and were and and the and the of to and ATP synthase ϵ and of ϵ the of the the of was the of the ϵ the the and of were The in was by the the of and to The in was by of by to the and of the the were by to and were and by of and the in to the the of the ϵ was The of was to The of to the N of ϵ in in of ϵ either and or and were to the the and the flavodoxin and of the and and the and mutants were the the and to and of ϵ mutant was the The of was and the to and mutants were the the and to and of ϵ and ϵ the of in ATP synthase and the role of the C-domain in a of proteins were both in the and of the The to of the the the to ATP to to the C-domain ATP synthase to the of a truncation in the of and to the of ϵ in ATP the ϵ the of the ϵ C-domain in of ATP were to or the of the the of the ϵ or the of ATP synthase and both and of the were in either the or the the of and was the and of the a a to by and a of the the ϵ to the The of ϵ The of the the of the of the of the the The and the ϵ by a of and the of the ϵ mutants the of ϵ The proteins were the and fusing the proteins a and a The of ϵ in and the domain of ϵ mutants a the of the mutants and to ϵ mutants in the 20-kDa flavodoxin or the 5-kDa the were of ϵ or were the C-domain or the were ϵ88-stop. of ϵ were the in a of in the of the of mutant ϵ to ϵ in of expressing F1F0 were the to grow the and the of ATP synthase and the cells to of the to the N of ϵ the of cells to of the the Cells the of either ϵ or the and mutants grow the the of ATP the of of the by the of the of All cells were and the were was were by the of and a of the synthase in a of ϵ of the the of the F1F0 or the were and the and was a of in the to a of was in the the the ϵ and a of in the the ϵ mutants the of of ϵ F1F0 and ϵ of were to by to were and ϵ to of were and to the by in the was the the was to the by in to the was of the ϵ proteins were the proteins were stable of of the of the and the were in the of F1F0 in was cells the were the of both F1F0 and the of and the a of the of ATP synthase in the The were to the of and the of a of the of the of F1F0 in the F1F0 a of the ATP synthase a of and was by the in of the the of and The of the 5-kDa to the N of ϵ either the or the of the the of ϵ or of the ATP of were to in in the or of and were to the of of of synthase were of in the were to the and the of were of in the were of in the were to the and the of in a of the 20-kDa flavodoxin to the N of ϵ a the to of the of the the ATP The was by of the was to the ATP synthase or the was the of ϵ was to the The ϵ subunit a of and ϵ to in ATP The of ϵ the of ATP synthase either ϵ or the by the of the Fd-ϵ mutant was by ATP a or of and ϵ the of were the of the to the the of of in the of the of the to the in the of the of the to the in a The of F1F0 the Fd-ϵ88-stop was to was by The and were and a in the ATP or and truncation of the ϵ ATP a of was to in the of ϵ mutants to the of F1F0 to a was by the of F1F0 of the and The in a of to the The Fd-ϵ and Fd-ϵ88-stop the and a the The a of the of the of were to in and and were and the ATP and the were to and F1F0 ϵ Fd-ϵ Fd-ϵ88-stop ϵ ϵ of ATP ATP ϵ ATP synthase by ATP ATP the of by F1F0 the of ATP ATP the the C-domain of ϵ the and F1F0 the or ATP ATP both the and mutant in the of of of in the of ATP a of to the mutant was the of ATP by the of the of ATP ATP The ATP ATP the and the mutant ATP were in the of of the of and to a to the of and cells of proteins were the to of the the of the in of the ϵ in of ATP was to the of was by the the was to by the to the C-domain or ATP the C-domain of ϵ to of the was both and were by the the the fusing the 20-kDa flavodoxin to the N of ϵ to the the C-domain to in and the C-domain ϵ to the ATP to ATP to in a to to either of of the or by fusing a to the N of ϵ to Cells expressing ATP synthase Fd-ϵ were unable to a of ATP synthase a of ATP of the of the The was to ATP synthase by and was to of the by the of was by ϵ of the by the ATP synthase ϵ the the the the ATP or the of in the in the the the of in the ATP synthase supported by the the ATP a of ATP of in in the of and the ATP synthase of by of to of ϵ of the C-domain the Fd-ϵ to the Fd-ϵ88-stop in a of the ATP was a of the C-domain in of ϵ and in of and or C-domain in were to in the of the The the of the C-domain of ϵ the role of the C-domain of ϵ in of ATP to to were truncation in in of the the of C-domain and the a of to the of of the in ATP synthase the Fd-ϵ88-stop and the and the in a the the the C-domain of ϵ in the the of and the the the or the of in the and and in ATP the C-domain in the Fd-ϵ88-stop or by the ATP and and a of in ATP synthase the ϵ or a in the and by the the the ϵ and the by of the in by and to by the in the of the and the of the of in the of ATP the to in The in of in the of of of ATP in ATP the of in of and The the of the binding of ATP to the binding the of the by and the of to the the and the the C-domain of ϵ of the C-domain in or of in the ϵ to of and of the ϵ in ATP ϵ in of ATP synthase of the to both in the and ϵ the the or the of ATP in the the ATP ϵ the and ATP the ATP the both and were the the the a of ATP and the ϵ the in the of to and the to the truncation of the C-domain of ϵ was of ATP a role ϵ of ATP in role ϵ in the and of ϵ ϵ a in ϵ F1F0 in the of ϵ and role and a the of the of supported by the of in in ATP ϵ in the of ATP synthase the the of ATP the of and and the in the in a to ATP the and The the of a of and the binding The a and of a of ATP the and the of the of the in both the binding of and and the and of ATP by the binding of The the a the and to ATP synthase and the to and the of The ϵ the of and to the a or a The of the by both and a domain the and a domain the The or in the of the the of The of ϵ in the ATP synthase of the and the in The in the in both in the the of the C-domain and to in the the and to the of the the of ATP ϵ the and ATP by in the of The ϵ subunit was to the ATP ATP synthase to either or of in by fusing proteins of to the of ϵ to the proteins was to by binding binding of the the of the by the in a the C-domain of ϵ a in ATP the by in the ATP the of the C-domain by of the and the to ϵ in the ATP synthase by ATP chitin binding domain flavodoxin chitin binding domain flavodoxin and to in ATP the of the of by a of and a of the of a 20-kDa to the N of ϵ and ATP The of was the C-domain was of the C-domain of ϵ in of ATP a of the of the C-domain to ATP The the of the were by All by and were by were by the of of ATP and were by of ATP were the The was by the of of and either or was The ϵ subunit was and were by and the and ϵ the domain of ϵ by of the a the ϵ mutants was of were and to the to the by in The was a and of the of and the was by and of The ϵ the ϵ mutants in the ATP synthase were and in the of ϵ the the was the of the ϵ of the the the and or the were and and the to and and were and and the and the of to and ATP synthase ϵ and of ϵ the of the the of was the of the ϵ the the and of were The in was by the the of and to The in was by of by to the and of the the were by to and were and by of and the in to the the of the ϵ was The of was to The of to the N of ϵ in in of ϵ either and or and were to the the and the flavodoxin and of the and and the and mutants were the the and to and of ϵ mutant was the The of was and the to and mutants were the the and to and were by All by and were by were by the of of ATP and were by of ATP were the The was by the of of and either or was The ϵ subunit was and were by and the and ϵ the domain of ϵ by of the a the ϵ mutants was of were and to the to the by in The was a and of the of and the was by and of The ϵ the ϵ mutants in the ATP synthase were and in the of ϵ the the was the of the ϵ of the the the and or the were and and the to and and were and and the and the of to and ATP synthase ϵ and of ϵ the of the the of was the of the ϵ the the and of were The in was by the the of and to The in was by of by to the and of the the were by to and were and by of and the in to the the of the ϵ was The of was to The of to the N of ϵ in in of ϵ either and or and were to the the and the flavodoxin and of the and and the and mutants were the the and to and of ϵ mutant was the The of was and the to and mutants were the the and to and of ϵ and ϵ the of in ATP synthase and the role of the C-domain in a of proteins were both in the and of the The to of the the the to ATP to to the C-domain ATP synthase to the of a truncation in the of and to the of ϵ in ATP the ϵ the of the ϵ C-domain in of ATP were to or the of the the of the ϵ or the of ATP synthase and both and of the were in either the or the the of and was the and of the a a to by and a of the the ϵ to the The of ϵ The of the the of the of the of the the The and the ϵ by a of and the ϵ mutants in the 20-kDa flavodoxin or the 5-kDa the were of ϵ or were the C-domain or the were ϵ88-stop. of ϵ were the in a of in the of the of mutant ϵ to ϵ in of expressing F1F0 were the to grow the and the of ATP synthase and the cells to of the to the N of ϵ the of cells to of the the Cells the of either ϵ or the and mutants grow the the of ATP the of of the by the of the of All cells were and the were was were by the of and a of the synthase in a of ϵ of the the of the F1F0 or the were and the and was a of in the to a of was in the the the ϵ and a of in the the ϵ mutants the of of ϵ F1F0 and ϵ of were to by to were and ϵ to of were and to the by in the was the the was to the by in to the was of the ϵ proteins were the proteins were stable of of the of the and the were in the of F1F0 in was cells the were the of both F1F0 and the of and the a of the of ATP synthase in the The were to the of and the of a of the of the of F1F0 in the F1F0 a of the ATP synthase a of and was by the in of the the of and The of the 5-kDa to the N of ϵ either the or the of the the of ϵ or of the ATP of were to in in the or of and were to the of of of synthase were of in the were to the and the of were of in the were of in the were to the and the of in a of the 20-kDa flavodoxin to the N of ϵ a the to of the of the the ATP The was by of the was to the ATP synthase or the was the of ϵ was to the The ϵ subunit a of and ϵ to in ATP The of ϵ the of ATP synthase either ϵ or the by the of the Fd-ϵ mutant was by ATP a or of and ϵ the of were the of the to the the of of in the of the of the to the in the of the of the to the in a The of F1F0 the Fd-ϵ88-stop was to was by The and were and a in the ATP or and truncation of the ϵ ATP a of was to in the of ϵ mutants to the of F1F0 to a was by the of F1F0 of the and The in a of to the The Fd-ϵ and Fd-ϵ88-stop the and a the The a of the of the of were to in and and were and the ATP and the were to and F1F0 ϵ Fd-ϵ Fd-ϵ88-stop ϵ ϵ of ATP ATP ϵ ATP synthase by ATP ATP the of by F1F0 the of ATP ATP the the C-domain of ϵ the and F1F0 the or ATP ATP both the and mutant in the of of of in the of ATP a of to the mutant was the of ATP by the of the of ATP ATP The ATP ATP the and the mutant ATP were in the of of the of and to of ϵ and ϵ the of in ATP synthase and the role of the C-domain in a of proteins were both in the and of the The to of the the the to ATP to to the C-domain ATP synthase to the of a truncation in the of and to the of ϵ in ATP the ϵ the of the ϵ C-domain in of ATP The were to or the of the the of the ϵ or the of ATP synthase and both and of the were in either the or the the of and was the and of the a a to by and a of the the ϵ to the The of ϵ The of the the of the of the of the the The and the ϵ by a of and the The ϵ mutants in the 20-kDa flavodoxin or the 5-kDa the were of ϵ or were the C-domain or the were ϵ88-stop. of ϵ were the in a of in the of the of mutant ϵ to ϵ in of expressing F1F0 were the to grow the and the of ATP synthase and the cells to of the to the N of ϵ the of cells to of the the Cells the of either ϵ or the and mutants grow the the of ATP the of of the by the of the of ϵ of the the of the F1F0 or the were and the and was a of in the to a of was in the the the ϵ and a of in the the ϵ mutants the of The was the the was to the by in to the was of the ϵ proteins were the proteins were stable of of the of the and the were in the of F1F0 in was cells the were the of both F1F0 and the of and the a of the of ATP synthase in the The were to the of and the of a of the of the of F1F0 in the F1F0 a of the ATP synthase a of and was by the in of the the of and The of the 5-kDa to the N of ϵ either the or the of the the of ϵ or of the ATP of the 20-kDa flavodoxin to the N of ϵ a the to of the of the the ATP The was by of the was to the ATP synthase or the was the of ϵ was to the The ϵ subunit a of and ϵ to in ATP The of ϵ the of ATP synthase either ϵ or the by the of the Fd-ϵ mutant was by ATP a or The of F1F0 the Fd-ϵ88-stop was to was by The and were and a in the ATP or and truncation of the ϵ ATP a of was to in the of ϵ mutants to the of F1F0 to a was by the of F1F0 of the and The in a of to the The Fd-ϵ and Fd-ϵ88-stop the and a the The a of the of the of ATP ATP ϵ ATP synthase by ATP ATP the of by F1F0 the of ATP ATP the the C-domain of ϵ the and F1F0 the or ATP ATP both the and mutant in the of of of in the of ATP a of to the mutant was the of ATP by the of the a to the of and cells of proteins were the to of the the of the in of the ϵ in of ATP was to the of was by the the was to by the to the C-domain or ATP the C-domain of ϵ to of the was both and were by the the the fusing the 20-kDa flavodoxin to the N of ϵ to the the C-domain to in and the C-domain ϵ to the ATP to ATP to in a to to either of of the or by fusing a to the N of ϵ to Cells expressing ATP synthase Fd-ϵ were unable to a of ATP synthase a of ATP of the of the The was to ATP synthase by and was to of the by the of was by ϵ of the by the ATP synthase ϵ the the the the ATP or the of in the in the the the of in the ATP synthase supported by the the ATP a of ATP of in in the of and the ATP synthase of by of to of ϵ of the C-domain the Fd-ϵ to the Fd-ϵ88-stop in a of the ATP was a of the C-domain in of ϵ and in of and or C-domain in were to in the of the The the of the C-domain of ϵ the role of the C-domain of ϵ in of ATP to to were truncation in in of the the of C-domain and the a of to the of of the in ATP synthase the Fd-ϵ88-stop and the and the in a the the the C-domain of ϵ in the the of and the the the or the of in the and and in ATP the C-domain in the Fd-ϵ88-stop or by the ATP and and a of in ATP synthase the ϵ or a in the and by the the the ϵ and the by of the in by and to by the in the of the and the of the of in the of ATP the to in The in of in the of of of the ϵ in ATP ϵ in of ATP synthase of the to both in the and ϵ the the or the of ATP in the the ATP ϵ the and ATP the ATP the both and were the the the a of ATP and the ϵ the in the of to and the to the truncation of the C-domain of ϵ was of ATP a role ϵ of ATP in role ϵ in the and of ϵ ϵ a in ϵ F1F0 in the of ϵ and role and a the of the of supported by the of in in ATP ϵ in the of a to the of and cells of proteins were the to of the the of the in of the ϵ in of ATP was to the of was by the the was to by the to the C-domain or ATP the C-domain of ϵ to of the was both and were by the the the fusing the 20-kDa flavodoxin to the N of ϵ to the the C-domain to in and the C-domain ϵ to the ATP ATP to ATP to in a to to either of of the or by fusing a to the N of ϵ to Cells expressing ATP synthase Fd-ϵ were unable to a of ATP synthase a of ATP of the of the The was to ATP synthase by and was to of the by the of was by ϵ of the by the ATP synthase ϵ the the the the ATP or the of in the in the the the of in the ATP synthase supported by the the ATP a of ATP of in in the of and the ATP synthase of by of to The of ϵ of the C-domain the Fd-ϵ to the Fd-ϵ88-stop in a of the ATP was a of the C-domain in of ϵ and in of and or C-domain in were to in the of the The the of the C-domain of ϵ the role of the C-domain of ϵ in of ATP to to were truncation in in of the the of C-domain and the a of to the of of the in ATP synthase the Fd-ϵ88-stop and the and the in a the the the C-domain of ϵ in the the of and the the the or the of in the and and in ATP the C-domain in the Fd-ϵ88-stop or by the ATP and and a of in ATP synthase the ϵ or a in the and by the the the ϵ and the by of the in by and to by the in the of the and the of the of in the of ATP the to in The in of in the of of of the ϵ in ATP ϵ in of ATP synthase of the to both in the and ϵ the the or the of ATP in the the ATP ϵ the and ATP the ATP the both and were the the the a of ATP and the ϵ the in the of to and the to the truncation of the C-domain of ϵ was of ATP a role ϵ of ATP in role ϵ in the and of ϵ ϵ a in ϵ F1F0 in the of ϵ and role and a the of the of supported by the of in in ATP ϵ in the of of the of the of and of the and of the of the of the and and of the of and of the of the were the and supported by a and the of

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.