First Report of Leaf Anthracnose Caused by <i>Phomopsis convolvuli</i> on Field Bindweed in Turkey
Notice bibliographique
Résumé
Field bindweed (Convolvulus arvensis L.; Convolvulaceae) is a troublesome perennial weed found among many important crops in the world (1). In May of 2007, dying field bindweed plants were found along the edge of a wheat (Triticum aestivum L.) field between Bafra and Taflan, Turkey (41°34.395′N, 35°52.215′E). Lesions on leaves were irregular and variable in size and dark black with green margins. Severely diseased leaves were wilted or dead. Fruiting bodies were not evident on field-collected material. Diseased tissue was surface disinfested and placed on moist filter paper in petri plates. Numerous pycnidia with alpha conidia were observed after 2 weeks. A fungus, designated 24-6, was isolated from the diseased leaves. Cultures on potato dextrose agar (PDA) were floccose with white mycelia and small black stromata. Alpha conidia from pycnidia on inoculated plants were biguttulate, one celled, hyaline, oblong to ellipsoid, and 7.0 to 12.8 × 3.0 to 5.5 μm (mean 10.0 × 3.9 μm). Neither beta conidia nor the teleomorph, Diaporthe sp., were observed on diseased tissue or in cultures. Morphology was consistent with that of Phomopsis convolvuli Ormeno-Nunez, Reeleder & A.K. Watson (2). Alpha conidia were harvested from 12-day-old cultures grown on PDA by brushing the surface of the colonies with a small paint brush, suspending the conidia in sterile distilled water, and filtering through cheesecloth. The conidia were then resuspended in sterile distilled water plus 0.1% polysorbate 20 to arrive at a concentration of 107 conidia/ml. Stems and leaves of seven plants at the 3- to 5-leaf stage were spray inoculated with 10 ml per plant of this aqueous suspension. Inoculated plants and two noninoculated plants were placed in a dew chamber at 24°C in darkness and continuous dew. After 48 h, plants from the dew chamber were moved to a greenhouse bench. Disease severity was evaluated 1 week after inoculation with a rating system based on a scale from 0 to 4, in which 0 = no symptoms, 1 = 1 to 25% necrosis, 2 = 26 to 50% necrosis, 3 = 51 to 75% necrosis, and 4 = 76 to 100% necrosis (2). The average disease rating on inoculated plants was 3.75. No disease was observed on noninoculated plants. P. convolvuli was reisolated from all inoculated plants. Comparison of the internal transcribed spacer (ITS) 1 and 2 sequences with available sequences of a vouchered P. convolvuli specimen (GenBank Nos. U11363, U11417; BPI 748009, FAU649) showed 192 of 193 and 176 of 179 identities, respectively, for the two regions. Nucleotide sequences for the ribosomal ITS regions (ITS 1 and 2, including 5.8S rDNA) were deposited in GenBank (Accession No. FJ710810), and a voucher specimen has been deposited with the U.S. National Fungus Collections (BPI 878927). To our knowledge, this is the second report in the world of leaf anthracnose on field bindweed caused by P. convolvuli. The first report was from Canada (3) of an isolate that was later patented for biological control of C. arvensis (4). References: (1) L. Holm et al. The World's Worst Weeds. University Press of Hawaii, Honolulu, 1977. (2) J. Ormeno-Nunez, et al. Can. J. Bot. 66:2228, 1988. (3) J. Ormeno-Nunez et al. Plant Dis. 72:338, 1988. (4) A. K. Watson et al. U.S. Patent 5,212,086, 1993.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
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Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».