Regulation of Systemic Acquired Resistance through the Interaction of Arabidopsis thaliana Transcription factors TGAI and TGA2 with NPRI
Notice bibliographique
Résumé
Arabidopsis thaliana is an established model plant system for studying plantpathogen \ninteractions. The knowledge garnered from examining the mechanism of \ninduced disease resistance in this model system can be applied to eliminate the cost and \ndanger associated with current means of crop protection. \nA specific defense pathway, known as systemic acquired resistance (SAR), \ninvolves whole plant protection from a wide variety of bacterial, viral and fungal \npathogens and remains induced weeks to months after being triggered. The ability of \nArabidopsis to mount SAR depends on the accumulation of salicylic acid (SA), the NPRI \n(non-expressor of pathogenesis related gene 1) protein and the expression of a subset of \npathogenesis related (PR) genes. NPRI exerts its effect in this pathway through \ninteraction with a closely related class of bZIP transcription factors known as TGA \nfactors, which are named for their recognition of the cognate DNA motif TGACG. \nWe have discovered that one of these transcription factors, TGA2, behaves as a \nrepressor in unchallenged Arabidopsis and acts to repress NPRI-dependent activation of \nPRJ. TGA1, which bears moderate sequence similarity to TGA2, acts as a transcriptional \nactivator in unchallenged Arabidopsis, however the significance of this activity is \nJ \nunclear. Once SAR has been induced, TGAI and TGA2 interact with NPRI to form \ncomplexes that are capable of activating transcription. Curiously, although TGAI is \ncapable of transactivating, the ability of the TGAI-NPRI complex to activate \ntranscription results from a novel transactivation domain in NPRI. This transactivation \ndomain, which depends on the oxidation of cysteines 521 and 529, is also responsible for \nthe transactivation ability of the TGA2-NPRI complex. Although the exact mechanism preventing TGA2-NPRI interaction in \nunchallenged Arabidopsis is unclear, the regulation of TGAI-NPRI interaction is based \non the redox status of cysteines 260 and 266 in TGAl. We determined that a \nglutaredoxin, which is an enzyme capable of regulating a protein's redox status, interacts \nwith the reduced form of TGAI and this interaction results .in the glutathionylation of \nTGAI and a loss of interaction with NPRl. \nTaken together, these results expand our understanding of how TGA transcription \nfactors and NPRI behave to regulate events and gene expression during SAR. \nFurthermore, the regulation of the behavior of both TGAI and NPRI by their redox \nstatus and the involvement of a glutaredoxin in modulating TGAI-NPRI interaction \nsuggests the redox regulation of proteins is a general mechanism implemented in SAR.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,002 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».