Abstract 3652: Sex Differences in CD200 and CD200R1 Expression in the Brain Following Experimental Stroke
Notice bibliographique
Résumé
Background and Purpose Emerging data suggests that stroke-induced inflammation significantly contributes to neuronal injury and clinical outcome. Local inflammatory responses following cerebral ischemia have highlighted a pivotal role for the innate immune system in the brain’s response to injury, including activation of microglia and macrophages. Cell-surface receptors (e.g. CD200R1) on microglia and other myeloid-derived cells directly interact with specific endogenous ligands (e.g. CD200) expressed on neurons and act to suppress pro-inflammatory signaling by maintaining microglia in an “inactive state”. Recent studies investigating the imbalance of CD200-CD200R1 signaling in models of neurodegenerative disease support the hypothesis that this interaction may also be disrupted following stroke. We investigated the role of the immune inhibitory receptor CD200R1, and its ligand, CD200, after experimental stroke in both male and female mice. Methods Gonadally intact male and female C57BL/6 mice were subjected to middle cerebral artery occlusion by reversible right MCA occlusion for 90min followed by 6h of reperfusion. Brains were harvested and processed for RNA and protein. mRNA and protein were analyzed for CD200 and CD200R1 expression using qPCR and Western blotting respectively in both stroke and sham mice. Immunohistochemistry was performed to identify the cellular source of CD200 and CD200R1 expression. Results At 6h post-reperfusion, decreased CD200 gene expression was seen in both sexes compared to sham. Western blot analysis demonstrated that CD200 was significantly decreased in males compared to females after stroke. CD200R1 gene levels were also downregulated in males compared to sham, however expression levels in females were increased after stroke. A relative 4-fold increase in CD200R1 protein levels was seen in stroke males compared to sham, in contrast CD200R1 expression in females decreased following stroke. Conclusions Early in the post-ischemic period, a marked difference between males and females in both CD200 and CD200R1 expression was seen, suggesting sex-dependent modulation of the stroke-induced inflammatory response. The decrease in CD200 ligand expression in males following stroke would be expected to lead to decreased CD200R1 activation, freeing microglial inhibition, thereby promoting a pro-inflammatory response. The increase in CD200R1 expression following stroke in males may act as a compensatory mechanism, potentially serving to enhance CD200-CD200R1 interactions between neurons and myeloid cells and thus suppress inflammation. These results provide a novel explanation for the larger cerebral infarcts seen in males, by a mechanism whereby stroke-induced disruption of the immunosuppressant interaction between CD200 and CD200R1 leads to worse outcome.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,006 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».