Markers of Ovarian Antral Follicular Development in Sheep: Differences Between Final (FW) and Penultimate (PW) Wave Pre-Ovulatory Follicles.
Notice bibliographique
Résumé
Treatment of non-prolific Western white-faced (WWF) ewes with prostaglandin F2alpha (PGF2alpha) and medroxy progesterone acetate (MAP) increased ovulation rate. The increase in ovulation rate was achieved largely by the addition of the ovulation of follicles from the penultimate (PW) follicular wave of the cycle to the expected ovulation of follicles from the final (FW) wave of the cycle. However, the number of lambs born after this treatment was not enhanced, suggesting that the PW follicles ovulating were not functionally viable. We were interested to compare the functional viability of PW and FW follicles. The objective of this study was to evaluate the expression of markers of vascularization and/or angiogenesis: vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), endothelial nitric oxide synthase (eNOS) and Factor VIII; a marker of gap junctional communication, connexin (Cx) 43; and a marker of cellular proliferation, proliferating cell nuclear antigen (PCNA) in preovulatory follicles obtained from the PW and FW in WWF ewes treated as described above. On day 8 of the estrous cycle, 15 ewes were given a single injection of PGF2alpha (15 mg i.m) and an intravaginal MAP sponge, which remained in place for 6 days. Thirty six to forty eight hours after sponge removal, ewes were ovariectomized by mid-ventral laparotomy. Ovaries that contain FW and/PW follicles from 7 ewes were fixed in Carnoy's solution for immunohistochemical detection of selected proteins. Computerized image analysis was used for quantification of protein expression. Granulosa and theca were isolated from FW and PW follicles from the remaining 8 ewes, suspended in PBS and stored at -80°C until RNA extraction. Messenger RNA levels were quantified using real-time PCR. Prostaglandin F2alpha and MAP treatment gave rise to preovulatory follicles emerging both from the FW and PW of the cycle; PW follicles had a longer (P<0.05) lifespan compared to FW follicles (7.1 ± 0.2 vs 3.0 ± 0.2). Protein expression for VEGF, eNOS, Cx43, PCNA and Factor VIII was greater in FW than in PW preovulatory follicles (P<0.05). For granulosa cells, Cx43 mRNA expression was greater (P<0.05) in FW than in PW preovulatory follicles. For theca cells, VEGF mRNA expression was greater (P<0.05) in FW than PW preovulatory follicles. In conclusion, expression of some markers of vascularization/ angiogenesis, gap junctional communication and cell proliferation appeared to be decreased in PW compared to FW preovulatory follicles. This suggests that although the treatment with PGF2alpha and MAP enhanced ovulation rate by extending the lifespan of PW follicles, these follicles were not as functionally viable as FW follicles. Therefore, this treatment provides a useful model to study aged antral follicles. (poster)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».