The Expression of the Transcription Factors SNAI1 and SNAI2 Throughout Pre-Implantation Development.
Notice bibliographique
Résumé
Mouse preimplantation development is characterized by two distinct morphological events: Compaction and Blastocyst Formation. Compaction is initiated at the 8-cell stage where the assembly of adherens (AJ) and tight junction proteins at the surface of the outer blastomeres begin the process of polarization of the outer cells and eventually provides a permeability seal in the outer cells of the early embryo. In concert with the formation of this seal, the Na/K ATPase establishes an ionic gradient across the trophectoderm (TE), which promotes the movement of fluid through aquaporins to form the blastocyst cavity. The blastocyst consists of two cell types: the TE, which will form the embryonic portion of the placenta, and the inner cell mass (ICM), which consists of the precursor cells that will become the embryo proper. Although we understand the role of many of the proteins involved in blastocyst formation, we have not elucidated how those proteins are regulated throughout preimplantation development. Of these, CDH1, an AJ associated protein, and the ATP1B1, a Na/K ATPase associated protein, are regulated by the transcription factors SNAI1 and SNAI2 in many cell types. SNAI1 and SNAI2 normally function to initiate epithelial-to-mesenchymal transition throughout mouse development. However, SNAI1 and SNAI2 are also oncogenes and aberrant expression of these proteins will promote cancer metastasis. My hypothesis is that Cdh1 and Atp1b1 are both regulated by SNAI1 and SNAI2 throughout mouse preimplantation development. Real-time RT-PCR was applied to determine the steady state mRNA levels of Snai1 and Snai2 throughout preimplantation development. Snai1 steady state mRNA expression increases at the 2-cell stage and then decreases until the 8-cell stage. The steady state mRNA levels returns to the 1-cell level in the blastocyst. Snai2 steady state mRNA levels decrease at the 2-cell stage during the maternal-to-zygotic transition. Expression begins to increase after the 4-cell stage and reaches its peak at the morula stage. Steady state mRNA then decreases at the blastocyst stage. Immunofluorescence was applied to detect SNAI1 and SNAI2 throughout preimplantation development and revealed that SNAI1 and SNAI2 are asymmetrically localized in each blastomere at the 2-cell and the 4-cell stage. SNAI1 and SNAI2 become exclusively distributed to only the outside cells of the developing embryo from the 8-cell stage onward. In the blastocyst, SNAI1 and SNAI2 are only detectable in the TE. SNAI1 and SNAI2 have a unique localization pattern throughout preimplantation development indicative of a role in lineage specification and TE differentiation. Loss-of-Function and Gain-of-Function studies will be applied to determine whether SNAI1 and SNAI2 play a role in TE differentiation and Cdh1 and Atp1b1 regulation. Together, these results provide further insight into the biochemical pathways that regulate blastocyst formation and provide new insight into the normal function of two oncogenes, SNAI1 and SNAI2 during preimplantation development. Research supported by the Canadian Institutes of Health Research. (poster)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».