Impacto da diversidade bacteriana sob a degradação clorotalonil no solo manejado com biochar
Notice bibliographique
Résumé
Impact of bacterial diversity in the chlorothalonil degradation on soil handled with biocharMicrobial diversity is generally considered for his role in key ecosystem processes, such as decomposition of organic matter and biogeochemical cycles.However, information about the impact of diversity on minor functions, such as degradation of xenobiotics is scant.We study from the approach of 'dilution to extinction', the role of diversity on the capacity of microbial community to degrade the chlorothalonil (organochlorine).We also studied the behavior of bacterial community after applying the pesticide in the soil with and without biochar.Microbial diversity of the soil natural (control) was artificially altered by dilution, forming a gradient of diversity (SN > 10 -1 > 10 -3 > 10 -6 ), followed by inoculation in sterile soil samples and subsequent restructuring (15 days).After of the community restructuring, the samples were handled with biochar (1% w/w) and treated with the chlorothalonil field dose.The behavior of the bacterial community was studied by PCR-DGGE and qPCR of the 16S rDNA gene through an experiment with cold molecule (no radiolabeled).While the CHT degradation capacity was studied by radiorespirometry ( 14 C-CHT).Initially, the community of bacteria was influenced by the diversity gradient obtained by dilution.The separation of bacterial groups showed very similar in the first three pre-application periods of the CHT (SN > 10 -1 -10 -3 > 10 -6 ).While in the period of 15 days, the group dynamic has changed (SN > 10 -1 > 10 -3 e 10 -6 ).During periods of 21 and 42 days, the profile bacterial community showed strong modification.The bacterial groups were more dispersed when only considered the CHT.Although, the ANOSIM analysis indicated no difference in samples with and without biochar, suggesting that chlorothalonil who has contributed the most in the dispersion of bacterial groups.In the period of 42 days, the community presented a positive response, being observed increases in the number of bands and Shannon-Weiner index in all treatments.This possibly due to less concentration of fungicide available in soil solution, thus reducing, the deleterious effects on the community.The qPCR dates showed no change in the number of copies of the 16S rDNA gene in all treatments.The removal of microbial strongly impacted the ability of the bacterial community to degrading chlorothalonil.Despite the ability to degrade not having been lost, the minimum change in diversity promoted high reduction in the rate of mineralization CHT.The dissipation of the CHT showed quick (D50 < 1 d) in all treatments, in addition, the formation of non-extractable 14 C-residues was one of the main mechanisms of dissipation of the CHT.From the degradation of chlorothalonil, three metabolites were detected.We conclude that modification by dilution of the bacterial diversity had a negative impact on the mineralization of chlorothalonil.And the formation of non-extractable residues consisted in the main CHT dissipation mechanism in both soils.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».