Impacto da diversidade bacteriana sob a degradação clorotalonil no solo manejado com biochar
Bibliographic record
Abstract
Impact of bacterial diversity in the chlorothalonil degradation on soil handled with biocharMicrobial diversity is generally considered for his role in key ecosystem processes, such as decomposition of organic matter and biogeochemical cycles.However, information about the impact of diversity on minor functions, such as degradation of xenobiotics is scant.We study from the approach of 'dilution to extinction', the role of diversity on the capacity of microbial community to degrade the chlorothalonil (organochlorine).We also studied the behavior of bacterial community after applying the pesticide in the soil with and without biochar.Microbial diversity of the soil natural (control) was artificially altered by dilution, forming a gradient of diversity (SN > 10 -1 > 10 -3 > 10 -6 ), followed by inoculation in sterile soil samples and subsequent restructuring (15 days).After of the community restructuring, the samples were handled with biochar (1% w/w) and treated with the chlorothalonil field dose.The behavior of the bacterial community was studied by PCR-DGGE and qPCR of the 16S rDNA gene through an experiment with cold molecule (no radiolabeled).While the CHT degradation capacity was studied by radiorespirometry ( 14 C-CHT).Initially, the community of bacteria was influenced by the diversity gradient obtained by dilution.The separation of bacterial groups showed very similar in the first three pre-application periods of the CHT (SN > 10 -1 -10 -3 > 10 -6 ).While in the period of 15 days, the group dynamic has changed (SN > 10 -1 > 10 -3 e 10 -6 ).During periods of 21 and 42 days, the profile bacterial community showed strong modification.The bacterial groups were more dispersed when only considered the CHT.Although, the ANOSIM analysis indicated no difference in samples with and without biochar, suggesting that chlorothalonil who has contributed the most in the dispersion of bacterial groups.In the period of 42 days, the community presented a positive response, being observed increases in the number of bands and Shannon-Weiner index in all treatments.This possibly due to less concentration of fungicide available in soil solution, thus reducing, the deleterious effects on the community.The qPCR dates showed no change in the number of copies of the 16S rDNA gene in all treatments.The removal of microbial strongly impacted the ability of the bacterial community to degrading chlorothalonil.Despite the ability to degrade not having been lost, the minimum change in diversity promoted high reduction in the rate of mineralization CHT.The dissipation of the CHT showed quick (D50 < 1 d) in all treatments, in addition, the formation of non-extractable 14 C-residues was one of the main mechanisms of dissipation of the CHT.From the degradation of chlorothalonil, three metabolites were detected.We conclude that modification by dilution of the bacterial diversity had a negative impact on the mineralization of chlorothalonil.And the formation of non-extractable residues consisted in the main CHT dissipation mechanism in both soils.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".