SRC Expression and Activity Regulate Mouse Blastocyst Formation.
Notice bibliographique
Résumé
The investigation of the mechanisms controlling preimplantation development is necessary to improve our assessment of embryonic development and to apply assisted reproductive technologies efficiently. The Na+/K+-ATPase plays a pivotal role during preimplantation development; the period between oocyte-sperm union and uterine wall invasion. Activity of the Na+/K+-ATPase establishes a trans-epithelial ionic gradient that facilitates the movement of water to form the fluid-filled blastocyst cavity. Reaching the blastocyst stage is necessary for implantation, and consequently, successful pregnancy. The Na+/K+-ATPase is implicated in regulating tight junction assembly and function and most recently in extracellular protein interactions. Signal transduction properties of the Na+/K+-ATPase have been discovered following binding of cardiotonic steroids (CTS), such as ouabain, to its α subunit leading to SRC activation. We hypothesize that pump activation induced by ouabain leads to SRC activation, resulting in the regulation of tight junction formation and function during mouse preimplantation development. To investigate this hypothesis, embryos were collected following application of superovulation methods to MF1 female mice. Preimplantaion embryos of the desired stage were subjected to CTS treatment or Src Family Kinase (SFK) inhibition followed by immmuofluorescence. Morula stage embryos were treated with 10-3 M, 10-5 M, 10-7 M, 10-9 M ouabain or bufalin to characterize affects of CTS treatment on blastocyst formation. Treatment with 10-5 M, 10-7 M, and 10-9 M CTS for 30 h did not significantly affect the proportion of morulae that progressed to the blastocyst stage. However, embryos treated with 10-3 M CTS did not progress to the blastocyst stage. Next experiments investigated the effects of treating blastocysts with 10-3 M, 10-4 M, and 10-5 M ouabain for 2 or 10 min to characterize influences of ouabain treatment on SRC phosphorylation by whole-mount immunofluorescence. Reduced Tyr418 phosphorylation after treatment with 10-3 M ouabain for 10 min was apparent when compared to control embryos. Next experiments treated morulae in PP2 (SFK blocker) for 18 h. Embryos treated with PP2 displayed a reversible blockade of development and did not cavitate using 20 μM, 30 μM, or 50 μM PP2. Morulae treated with SU6656, a more potent and specific SFK inhibitor, for 18hr also displayed an inability to cavitate using 1, 5, 10 μM doses. RT-PCR analysis was used to detect mRNAs encoding all SFKs during preimplantation development. mRNAs encoding SRC and YES were detectable at the blastocyst stage while mRNAs encoding FGR, LCK, LYN, FYN, HCK, and BLK were not present in blastocysts. These studies contribute to our understanding of the mechanisms controlling preimplantation development. Activation of SRC through phosphorylation of tyrosine 418 is necessary for mouse blastocyst formation. The presence of a ouabain-induced Na+/K+-ATPase signaling transduction pathway via SRC was revealed in the mouse blastocyst. Future experiments will investigate the effect of SFK inhibition on trophectoderm tight junction function. Research supported by operating grant from the Canadian Institutes of Health Research. (poster)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».