Local Irradiation of Recipient Piglet Testes with Doses as Low as One Gray Completely Suppresses the Endogenous Spermatogenesis in Preparation for Germ Cell Transplantation.
Notice bibliographique
Résumé
Preparation of the recipient testes can enhance the outcome of germ cell transplantation. Whether neonatal pig gonocytes have stem cell potential has not been shown. Therefore, the objectives of the present study were 1) to examine the effects of neonatal piglet testis irradiation on testis development and subsequent endogenous spermatogenesis, and 2) to investigate the potential of donor neonatal piglet gonocytes in establishing spermatogenesis after homologous transplantation into irradiated recipient testes. Nine-day-old piglets underwent daily local irradiation of testes using fractionated gamma-rays at doses of 0 (control), 1, 2 or 3 Gray (n = 6 piglets/group) for 3 consecutive days. Two months after irradiation, half of the pigs in each group were sacrificed and the testes examined. At the same time, the remaining pigs in each group were used as recipients for donor neonatal piglet testis cells. The donor cells were injected, through the rete testis under ultrasound guidance, into one testis of each irradiated and non-irradiated recipient animal, and the contra-lateral testis was injected with saline as an internal control. Two months after transplantation (i.e., 4 months after irradiation), pigs were sacrificed and the testes collected for examination. Compared with the control (0 Gray), at both 2- and 4 months post-irradiation, the testis weight indices from the group of pigs undergoing daily irradiation doses of 3 Gray were smaller, seminiferous tubule density from 2- and 3-Gray groups were lower, and tubule diameter in all irradiated testes were also lower (P < 0.05). Two months after irradiation with doses of 2 or 3 Gray, the relative number of germ cells per 1,000 Sertoli decreased (P < 0.05). At 4 months post-irradiation, spermatogenic differentiation up to elongating spermatids was observed in all non-irradiated testes, and while no spermatogenic development was found in any of the irradiated testes injected with only saline; after gonocyte transplantation, a small number of tubules in the groups of 1- and 2-Gray contained spermatogenesis up to elongating and round spermatids. In conclusion, local irradiation of recipient piglet testes with as low as 1 Gray (for 3 days) could completely suppress the endogenous spermatogenesis, and gonocyte transplantation into irradiated recipients led to initiation of spermatogenesis. (poster)
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».