Local Irradiation of Recipient Piglet Testes with Doses as Low as One Gray Completely Suppresses the Endogenous Spermatogenesis in Preparation for Germ Cell Transplantation.
Bibliographic record
Abstract
Preparation of the recipient testes can enhance the outcome of germ cell transplantation. Whether neonatal pig gonocytes have stem cell potential has not been shown. Therefore, the objectives of the present study were 1) to examine the effects of neonatal piglet testis irradiation on testis development and subsequent endogenous spermatogenesis, and 2) to investigate the potential of donor neonatal piglet gonocytes in establishing spermatogenesis after homologous transplantation into irradiated recipient testes. Nine-day-old piglets underwent daily local irradiation of testes using fractionated gamma-rays at doses of 0 (control), 1, 2 or 3 Gray (n = 6 piglets/group) for 3 consecutive days. Two months after irradiation, half of the pigs in each group were sacrificed and the testes examined. At the same time, the remaining pigs in each group were used as recipients for donor neonatal piglet testis cells. The donor cells were injected, through the rete testis under ultrasound guidance, into one testis of each irradiated and non-irradiated recipient animal, and the contra-lateral testis was injected with saline as an internal control. Two months after transplantation (i.e., 4 months after irradiation), pigs were sacrificed and the testes collected for examination. Compared with the control (0 Gray), at both 2- and 4 months post-irradiation, the testis weight indices from the group of pigs undergoing daily irradiation doses of 3 Gray were smaller, seminiferous tubule density from 2- and 3-Gray groups were lower, and tubule diameter in all irradiated testes were also lower (P < 0.05). Two months after irradiation with doses of 2 or 3 Gray, the relative number of germ cells per 1,000 Sertoli decreased (P < 0.05). At 4 months post-irradiation, spermatogenic differentiation up to elongating spermatids was observed in all non-irradiated testes, and while no spermatogenic development was found in any of the irradiated testes injected with only saline; after gonocyte transplantation, a small number of tubules in the groups of 1- and 2-Gray contained spermatogenesis up to elongating and round spermatids. In conclusion, local irradiation of recipient piglet testes with as low as 1 Gray (for 3 days) could completely suppress the endogenous spermatogenesis, and gonocyte transplantation into irradiated recipients led to initiation of spermatogenesis. (poster)
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".