GnRH Agonist-Induced Up-Regulation of Estrogen Receptors in Bovine Endometrium.
Notice bibliographique
Résumé
Endometrium is one of the primary target sites for the action of ovarian steroids estrogen and progesterone. These hormones exert their action through their corresponding nuclear receptors. Estrogen receptors(ER α and ER β) are shown to be present in bovine endometrium during follicular and luteal phases of estrous cycle, bovine ovaries and placentomes, and ER α has been reported to be a predominant estrogen receptor in endometrium. Uterine functions such as cellular proliferation, gamete transport and implantation are influenced by estrogen by acting through its corresponding receptors. We, most recently demonstrated the presence of Gonadotropin releasing hormone receptor (GnRH-R) in bovine endometrium at both mRNA and protein levels, during both follicular and luteal phases of the estrous cycle. GnRH is widely used in bovine reproductive management; including estrous and ovulation synchronization, induction of ovulation, post partum cyclicity, cystic ovarian disease and to increase pregnancy rates. However, there is clear lack of information on its local modulatory role in endometrium. We find the co-existence of GnRH-R and estrogen receptors as interesting as there are prior reports about ligand independent activation of steroid hormone receptors. Whether GnRH through its receptors could regulate estrogen receptors in normal endometrium is still not known. This study for the first time examined the GnRH induced regulation of ER α and ER β in bovine endometrium. Uteri belonging to follicular and luteal phases of the estrous cycle were collected from the local abattoir and transported to lab within one hour to obtain endometrial explants. 100 mg of explant tissues were cultured under humidified atmosphere and after 20 h incubation, treated with different doses of GnRH agonist - buserelin (0, 200, 500, and 1000 ng/mL), GnRH antagonist - antide (500 ng/mL) and a combination of antide (500ng/mL) and buserelin (200ng/mL) for 6 h. Two µg of total RNA extracted from each treatment was reverse transcribed using commercial available kit and the mRNA levels of ER α and ER β were assessed by a semi-quantitative RT-PCR and using gene specific primers. G3PDH was used as an internal control. Optical intensity of individual bands was analyzed by Scion imaging beta and statistically analyzed by comparing to control, and by using student t test. This study revealed that GnRH at the dose of 200ng/mL up-regulated ER α mRNA in endometrial explants and this effect was more pronounced (P≤0.05) in the luteal phase endometrium. mRNA levels of ER β were not altered during the experimental period. GnRH induced up regulation of ER α could have potential implications on reproductive process such as gamete transport, cellular proliferation, uterine receptivity, implantation and maintenance of normal physiological status of the uterus and increase our understanding of the molecular basis of reproductive functions at the endometrial level.
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Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,002 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».