EXPRESSION AND IMMUNOLOCALIZATION OF GNRH RECEPTORS IN BOVINE UTERUS AND OVIDUCTS
Notice bibliographique
Résumé
Gonadotropin-releasing hormone (GnRH) is a decapeptide, synthesised and secreted by hypothalamic neurones and acts as a central regulator of mammalian reproduction. This hormone exerts its action by binding to transmembrane G protein - coupled receptors on pituitary gonadotrophs and controls synthesis and release of follicle stimulating hormone (FSH) and leutinizing hormone (LH). FSH and LH further regulate the processes of gametogenesis and gonadal steroidogenesis. Recently, extrapituitary GnRH receptors (GnRH-R) have been demonstrated in ovaries, testes, prostrate, placenta and uterus in different mammalian species and GnRH acts in an autocrine and or paracrine manner in these reproductive tissues and modulates vital biological processes including gonadal steroidogenesis, cellular proliferation and apoptosis. GnRH-R mRNA has also been shown to be present in bovine ovaries (Follicle and corpus luteum) and normal and neoplastic human endometrium / endometrial cells. We for the first time studied the presence of GnRH-R mRNA and GnRH-R protein in bovine uterus and oviducts in the follicular and luteal phases of the estrous cycle and further demonstrated that these receptors are localized in endometrial and oviductal epithelial cells. To our knowledge this is the first report demonstrating presence of GnRH-R mRNA and protein in the oviducts in any mammalian species. We used RT-PCR with gene - specific primers to detect the expression of GnRH-R mRNA and used G3PDH as housekeeping gene. Immunobloting and immunohistochemistry techniques with receptor specific monoclonal antibodies were employed to study the expression of GnRH-R protein in these tissues and bovine pituitaries were used as positive control in these experiments. In this study GnRH-R mRNA and protein were expressed at expected molecular weights of 920bp and 60kD, respectively. Densitometry analysis performed on protein expression further revealed that there was no variation in the expression patterns of GnRH-R in uterus and oviducts when compared either to one another or to the positive pituitary control. Through immunohistochemistry experiments the presence of these receptors could further be localized to endometrial and oviductal epithelial cells in both the follicular and luteal phases of the estrous cycle and 30–40% of pituitary cells showed immunoreactivity to the GnRH-R monoclonal antibody used in the experiments. GnRH binds to its receptors on the pituitary gonadotrophs and central regulatory role of this hormone in mammalian reproduction is well established. GnRH has also been shown to have antiproliferative effect in endometrial carcinomas and endometriosis. But the presence of GnRH receptors in normal uterus and oviducts is intriguing and is an indication that equally functional system might exist in these tissues. There is clear lack of information on local physiological actions of this hormone in these reproductive tissues and therefore it would be imperative to further investigate the modulatory role of this system in mammalian reproductive processes such as gamete transport, fertilization, uterine receptivity, early embryonic development and implantation with an overall objective to improve declining reproductive efficiency in dairy cows. In addition, these studies could also add to the existing knowledge in the field of reproductive biology. (platform)
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,001 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».