Fundamental Investigation of Inkjet Deposition and Physical Immobilization of Horseradish Peroxidase on Cellulosic Substrates
Notice bibliographique
Résumé
In this study, novel bio-inks formulated with horseradish peroxidase, HRP, and some additives were successfully developed for piezoelectric inkjet application. The optimized bio-ink formulation had a reliable jetting performance and maintained the biofunctionality before and after printing. The bio-ink also demonstrated a good storage life for up to 40 days at 4 oC with a negligible loss of biofunctionality. However, it was observed that some additives used in the bio-ink for obtaining necessary operational characteristics had detrimental effects on the enzyme activity. Especially, it was found that various viscosity modifiers typically used in commercial inkjet inks significantly impaired HRP activity prior to printing. Sodium Carboxymethyl Cellulose was shown to be an effective viscosity modifier that had no adverse effect on the biological activity of the HRP enzyme.\n\nUsing a confocal scanning fluorescent microscope, a method for characterizing the spatial distribution of the active enzyme within the cellulosic paper substrates after inkjet printing was developed. Interestingly, it was found that the active printed HRP enzyme was mostly located in the cell walls of the cellulosic fibers instead of near the pigments or fillers. \n\nIn an effort to better understand the fundamental interactions between the enzyme and the immobilization substrates, HRP adsorption isotherms on various substrate surfaces were obtained using the depletion method. The substrates included not only pulp fibers with varying degree of hydrophobicity and pigment and latex (the key materials used in papermaking), but also other types of cellulosic fibers of different morphologies, crystallinities, porosities, or surface charge densities. The influence on enzyme adsorption and inactivation behaviour of these substrates was compared with that of polystyrene beads (dialysed), which has been well studied in the literature. Results from this thesis indicated that hydrophobic interactions between the enzyme and the substrate surfaces had a major impact on the HRP adsorption behavior, while electrostatic interactions played a minor role. However, strong hydrophobic interactions could lead to enzyme inactivation. Research findings from this study suggested that cellulosic pulp fibers could be tailor-made into excellent enzyme immobilization supports by using existing fiber surface modification techniques.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».