Longitudinal evaluation of serum markers of endothelial cell dysfunction and haemostasis in treated antiphospholipid syndrome and healthy pregnancies
Notice bibliographique
Résumé
Antiphospholipid syndrome (APS) pregnancies are characterised by placental insufficiency leading to recurrent fetal deaths and pre-eclampsia, placental abruption and/or maternal thrombotic events. In vitro antiphospholipid antibodies (aPLs) can produce endothelial cell activation (ECA). We hypothesised that the placental dysfunction seen in APS pregnancies was related to ECA in the maternal circulation which may predispose to poor placentation with failed endovascular trophoblast invasion into the maternal spiral arteries of the placental bed. Resultant decidual vasculopathy, characteristic of growth restricted and pre-eclamptic pregnancies, may lead to the above pregnancy complications. We collected clinical data and sequential blood samples from 22 primary APS pregnancies and 22 control pregnancies. All APS pregnancies received aspirin and low molecular weight (LMW) heparin (Fragmin). Three APS women with previous cerebral thrombotic events, who developed cerebral symptoms on LMW heparin, were prescribed warfarin from the 2nd trimester. The control pregnancies were untreated. We measured the following markers of haemostatic activation and ECA by ELISA: prothrombin fragments 1+2 (PF 1+2), D-dimers, plasminogen activator inhibitor type 1 (PAI-1), tissue plasminogen activator (tPA) and soluble intercellular adhesion molecule (sol-ICAM) and soluble thrombomodulin (sTM), a marker of endothelial cell damage. Statistical analysis was conducted on logged values (log-normal distribution) and results presented as geometric means. The gestational ages were rounded into 4-week groups to allow analysis of repeated measures using generalised estimating equations (GEE). Clinical outcome: All control pregnancies were uncomplicated. In the APS group, there were 3 cases of intra-uterine growth restriction of the fetuses, 1 case of placental abruption, 2 women developed pre-eclampsia and there were 4 preterm deliveries. Of the maternal complications 5 women developed transient ischaemic attacks (TIAs) but not completed cerebral events and one woman developed thrombotic microangiopathy in pregnancy. There was no difference in the levels of ICAM or PAI-1 whether analysed by direct comparison, effect of treatment or according to outcome; t-PA levels were significantly higher in APS group (p=0.002); sTM levels were significantly lower in the APS group (p=0.02); PF 1+2 levels increased during pregnancy, increased less in the APS group (not statistically significant p=0.08) but was significantly lower in those receiving warfarin and in the group with maternal complications (p=0.018). D-dimer levels were significantly lower in those receiving warfarin (p=0.042). Conclusion: There is no evidence of generalised ECA in treated APS pregnancies compared to healthy controls. Levels of fibrinolytic activity were higher in APS pregnancies. A significant number of patients (23%) had TIAs on heparin but those that switched to warfarin had no further events. This may be because warfarin had a significantly greater effect on suppressing coagulation activation than LMW heparin
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,004 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».