Abstract 5176: Cell free malignant ascites fluid facilitates gastric adenocarcinoma peritoneal metastasis
Notice bibliographique
Résumé
Abstract Gastric adenocarcinoma is the fastest rising malignancy in North America. It is commonly associated with malignant ascites (MA), the pathological accumulation of fluid containing cancer cells in the peritoneum. Peritoneal metastasis is the most common site of gastric cancer (GC) progression after curative intent surgery and is the leading cause of death. Upon peritoneal dissemination, the malignant process is deemed non-curative as it is rarely amenable to surgical resection and chemotherapeutic regimens are simply palliative. We hypothesize that cell-free MA increases the potential of GC cells to worsen peritoneal metastasis. Gastric adenocarcinoma cell lines (human: MKN-45, SNU-5, KATO III, OKAJIMA; murine NCC-S1, NCC-S1M) were incubated with cell-free MA and their metastatic ability assessed with static in vitro adhesion assays, as well as migration assays. A novel ex vivo peritoneal metastasis model further corroborated the in vitro results, where cancer cell adhesion to stripped human peritoneum was assessed by co-incubation with non- and pre-stimulated cancer cells. Gross liver metastasis was monitored over several weeks after in vivo intra-peritoneal and intra-splenic injections, whereby C57BL/6 mice were inoculated with control media or stimulated NCC-S1M cells. Incubation of human GC cells and/or human peritoneal mesothelial cells (HPMC) with cell-free MA resulted in a significant three to five-fold increase of GC cell adhesion to HPMC compared to non-stimulated condition (P<0.05), as measured by inverted fluorescent microscopy. In addition, the murine GC cells incubated with MA showed a significant eight-fold increase on average (P<0.05) in GC cell adhesion to HPMC compared to non-stimulated condition. Cell-free MA was shown to significantly enhance ex vivo SNU-5 cell adhesion to stripped human peritoneum by a two to four-fold increase (P<0.05) compared to non-stimulated condition. Liver metastases were visible in mice that received in vivo injections of ascites-stimulated NCC-S1M cancer cells by day 25, yet not in mice inoculated with control media. Several factors (ANG-2, HGF, ICAM-1, IL-8, TIMP-2, uPAR, VEGF, NAP-2, MIF) were shown to be upregulated in MA samples compared to a cirrhotic ascites control, using a multiplex ELISA. In particular, VEGF was upregulated 11 to 25-fold, and MIF two to 12-fold. The results demonstrated that MA plays a significant role in facilitating GC cell adhesion to peritoneal mesothelia, an important early step in the peritoneal metastatic cascade. MA must therefore provide an environment that supports tumour growth and spread. A more comprehensive understanding of the molecular network is essential to determine the role of cell free MA fluid in GC progression, allowing for the identification of potential therapeutic targets for this aggressive malignancy. Citation Format: Chantelle A. Janeiro, Vivian Stavrakos, Malak Alzahrani, Roni F. Rayes, France Bourdeau, Betty Giannias, Nicholas Bertos, Veena Sangwan, Jonathan Cools-Lartigue, Jonathan D. Spicer, Lorenzo E. Ferri. Cell free malignant ascites fluid facilitates gastric adenocarcinoma peritoneal metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5176.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,001 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,001 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».