Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
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Résumé
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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La notice
- Revue
- Journal of Extracellular Vesicles
- Thématique
- Extracellular vesicles in disease
- Domaine
- Biochemistry, Genetics and Molecular Biology
- Établissements canadiens
- Hospital for Sick ChildrenSickKids FoundationOkanagan University CollegeUniversity of British Columbia, Okanagan CampusPrincess Margaret Cancer CentreCanada's Michael Smith Genome Sciences CentreToronto General HospitalUniversity of TorontoOttawa HospitalUniversity Health NetworkMcGill University Health CentreSimon Fraser UniversityMcGill UniversityUniversity of OttawaUniversity of British ColumbiaCentre hospitalier de l'Université LavalAtlantic Cancer Research InstituteMount Allison University
- Organismes subventionnaires
- National Institute of General Medical SciencesNational Cancer InstituteNational Institute on Drug AbuseNational Institute of Mental HealthNational Heart, Lung, and Blood InstituteNational Institute on AgingMedical Research CouncilInstitut National Du CancerInstitut National de la Santé et de la Recherche MédicaleNational Institute of Diabetes and Digestive and Kidney DiseasesBiotechnology and Biological Sciences Research CouncilNational Center for Advancing Translational SciencesFondation ARC pour la Recherche sur le CancerAgence Nationale de la RechercheNational Institutes of HealthCancer Research UKSidaction
- Mots-clés
- Extracellular vesiclesVesicleMicrovesiclesExtracellularPosition (finance)Statement (logic)ChemistryBiologyCell biologyPolitical scienceBiochemistryLawBusiness
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