MP03-10 EFFECT OF A BACTERIAL URINARY INFECTION ISOLATE ON CALCIUM OXALATE CRYSTAL ADHERENCE TO RENAL EPITHELIAL CELLS
Notice bibliographique
Résumé
You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology I (MP03)1 Apr 2019MP03-10 EFFECT OF A BACTERIAL URINARY INFECTION ISOLATE ON CALCIUM OXALATE CRYSTAL ADHERENCE TO RENAL EPITHELIAL CELLS Jennifer Bjazevic*, Kaitlin F. Al, Jaswanth Gorla, Hassan Razvi, and Jeremy P. Burton Jennifer Bjazevic*Jennifer Bjazevic* More articles by this author , Kaitlin F. AlKaitlin F. Al More articles by this author , Jaswanth GorlaJaswanth Gorla More articles by this author , Hassan RazviHassan Razvi More articles by this author , and Jeremy P. BurtonJeremy P. Burton More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000554942.41700.d3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: There is growing evidence to support a role of urinary bacteria in the development of calcium stone disease; however, the mechanisms by which urinary bacteria may influence calcium stone formation remain to be elucidated. Both osteopontin (OPN) and zinc (Zn) have been shown to play a role in calcium oxalate (CaOx) urolithiasis and have also been demonstrated to be involved in bacterial pathogenesis. We aimed to examine the effect of non-urease producing bacteria isolated from a urinary tract infection, as well as both OPN and Zn, on the adherence of calcium oxalate crystals to renal epithelial cells. METHODS: HEK293 and MDCK renal epithelial cells were grown to 90% confluence on culture plates. Cells were exposed to a non-urease producing strain of Escherichia coli (UTI89) for 20 minutes at 37C (103 CFU). The cells were then incubated with CaOx crystal suspension (0.5mg/mL) in artificial urine with or without the addition of OPN (0.1 μg/ml) or zinc chloride (500 μg/L), for 20 minutes at 37C. Unattached crystals were washed free with culture media. The adherence of calcium oxalate crystals was determined with birefringence microscopy and quantified by pixel intensity with MatLab. RESULTS: Microscopy following treatment exposure revealed live cells in all treatment groups, and bacterial rods visible in groups treated with UTI89. In the MDCK cells, exposure to UTI89 significantly increased crystal adherence (p<0.001, FIgure 1). Exposure to OPN and Zn were noted to have opposite effects in UTI89 treated cells, resulting in attenuated and enhanced crystal adherence respectively (p<0.001, Figure 1). Similar trends were observed in the HEK293 cells; however, these results did not reach statistical significance. CONCLUSIONS: These findings suggest that non-urease producing E. coli impacts CaOx crystal adherence, and both Zn and OPN may have a novel role in this process. While Zn and OPN have previously been shown to be involved in both CaOx urolithiasis and bacterial pathogenesis; further investigation is required in order to delineate the potential mechanisms by which urinary pathogens alter crystal adherence, and the precise role that both Zn and OPN may play in this process. Source of Funding: AUA Urology Care Foundation Research Scholar Award London, Canada© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e24-e24 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jennifer Bjazevic* More articles by this author Kaitlin F. Al More articles by this author Jaswanth Gorla More articles by this author Hassan Razvi More articles by this author Jeremy P. Burton More articles by this author Expand All Advertisement PDF downloadLoading ...
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,001 | 0,001 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,001 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,001 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,025 | 0,003 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».