MP03-10 EFFECT OF A BACTERIAL URINARY INFECTION ISOLATE ON CALCIUM OXALATE CRYSTAL ADHERENCE TO RENAL EPITHELIAL CELLS
Bibliographic record
Abstract
You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology I (MP03)1 Apr 2019MP03-10 EFFECT OF A BACTERIAL URINARY INFECTION ISOLATE ON CALCIUM OXALATE CRYSTAL ADHERENCE TO RENAL EPITHELIAL CELLS Jennifer Bjazevic*, Kaitlin F. Al, Jaswanth Gorla, Hassan Razvi, and Jeremy P. Burton Jennifer Bjazevic*Jennifer Bjazevic* More articles by this author , Kaitlin F. AlKaitlin F. Al More articles by this author , Jaswanth GorlaJaswanth Gorla More articles by this author , Hassan RazviHassan Razvi More articles by this author , and Jeremy P. BurtonJeremy P. Burton More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000554942.41700.d3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: There is growing evidence to support a role of urinary bacteria in the development of calcium stone disease; however, the mechanisms by which urinary bacteria may influence calcium stone formation remain to be elucidated. Both osteopontin (OPN) and zinc (Zn) have been shown to play a role in calcium oxalate (CaOx) urolithiasis and have also been demonstrated to be involved in bacterial pathogenesis. We aimed to examine the effect of non-urease producing bacteria isolated from a urinary tract infection, as well as both OPN and Zn, on the adherence of calcium oxalate crystals to renal epithelial cells. METHODS: HEK293 and MDCK renal epithelial cells were grown to 90% confluence on culture plates. Cells were exposed to a non-urease producing strain of Escherichia coli (UTI89) for 20 minutes at 37C (103 CFU). The cells were then incubated with CaOx crystal suspension (0.5mg/mL) in artificial urine with or without the addition of OPN (0.1 μg/ml) or zinc chloride (500 μg/L), for 20 minutes at 37C. Unattached crystals were washed free with culture media. The adherence of calcium oxalate crystals was determined with birefringence microscopy and quantified by pixel intensity with MatLab. RESULTS: Microscopy following treatment exposure revealed live cells in all treatment groups, and bacterial rods visible in groups treated with UTI89. In the MDCK cells, exposure to UTI89 significantly increased crystal adherence (p<0.001, FIgure 1). Exposure to OPN and Zn were noted to have opposite effects in UTI89 treated cells, resulting in attenuated and enhanced crystal adherence respectively (p<0.001, Figure 1). Similar trends were observed in the HEK293 cells; however, these results did not reach statistical significance. CONCLUSIONS: These findings suggest that non-urease producing E. coli impacts CaOx crystal adherence, and both Zn and OPN may have a novel role in this process. While Zn and OPN have previously been shown to be involved in both CaOx urolithiasis and bacterial pathogenesis; further investigation is required in order to delineate the potential mechanisms by which urinary pathogens alter crystal adherence, and the precise role that both Zn and OPN may play in this process. Source of Funding: AUA Urology Care Foundation Research Scholar Award London, Canada© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e24-e24 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jennifer Bjazevic* More articles by this author Kaitlin F. Al More articles by this author Jaswanth Gorla More articles by this author Hassan Razvi More articles by this author Jeremy P. Burton More articles by this author Expand All Advertisement PDF downloadLoading ...
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.025 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".