Chemical Profiling of Five Canadian Haskap Berry Varieties and the Roles of their Phenolic Extracts in Modulating Cellular Stress in Human Fibroblasts
Notice bibliographique
Résumé
Interest in plant phenolic structure(s) and their applications has grown due to their potent antioxidant and nutraceutical potential. Many plant species produce phenolics for a variety of functions, with high levels of these compounds often found in berry fruits. When applied to cells and organisms, berry fruit phenolics have shown anti-aging, anticancer, and anti-inflammatory activities. Haskap berries (Lonicera caerulea) are an edible honeysuckle species that contain very high levels of phenolics when compared to other commercial fruits. In this study, the chemical and physicochemical composition of five Canadian haskap berry varieties (Aurora, Blizzard, Honey Bee, Indigo Gem, and Tundra) was determined, with a focus on phenolic analysis and the in vitro health-promoting potential of these compounds. Total phenolic chromatographic indices (TPCI) using high performance liquid chromatography with photodiode array detection (HPLC-PDA) identified anthocyanins as the major phenolic subclass with cyanidin-3-O-glucoside, cyanidin-3,5-O-diglucoside, and cyanidin-3-O-rutinoside being the most prevalent. HPLC-tandem mass spectrometry (HPLC-MS/MS) was also employed to identify haskap phenolic structures including three previously unreported phenolic compounds. In vitro free radical scavenging assays showed that these haskap varieties had higher radical scavenging abilities than many other fruits (e.g. blueberry) with the Tundra variety demonstrating the highest capacity. These experiments led to selection of the Tundra variety for use in tissue culture assays due to its high phenolic content and free radical scavenging abilities. Phenolic extracts and fractions from Tundra haskap berries were produced using solid phase extraction, so as to separate phenolic subclasses for comparative cell line treatments in vitro. In tissue culture assays, phenolic extracts from the Tundra variety slowed the growth of fibroblasts isolated from healthy donors (2DD) and immortalized (NB1 hTERT) fibroblasts without inducing cell death, indicating potential biological impacts similar to resveratrol, a fruit phenolic known to promote cellular health. Tundra phenolic fractions also increased cellular protein and activity levels of Sirtuin-1 (SIRT1), decreased levels of the pro-inflammatory phospho-nuclear factor kappa B (phospho-NF-κB p65) and the phosphorylated mammalian target of rapamycin (phospho-mTOR), and increased nuclear factor erythroid 2 (Nrf2). These observations are significant as these activities have been linked to slowed aging, improved intracellular radical scavenging (resistance to oxidative stress), and decreased inflammatory responses. Tundra phenolic fraction activities were shown to rely primarily on SIRT1 by employing lentiviral and siRNA SIRT1 knockdown cells, with additional SIRT1-independent mechanism(s) related to Nrf2 upregulation. These fractions also showed the ability to decrease transcript levels of select pro-inflammatory cytokines and antioxidant enzymes, demonstrating further anti-inflammatory activities along with decreased requirements for cellular antioxidant mechanisms. These experimental results indicated that Saskatchewan-bred haskaps have the potential to promote health in vitro by mediating cellular stress responses and may be candidates for use as nutraceutical supplements.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,001 | 0,001 |
| Études des sciences et des technologies | 0,001 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».