Effects of Ultrasonication on the Antioxidant and Anti-diabetic Properties of Hydrolyzed Oat Proteins
Notice bibliographique
Résumé
The method of protein extraction from foods can affect yields, compositions, and functionalities of the isolated proteins.The first objective of this work was to determine the effects of ultrasonic treatments on protein yields and polypeptide compositions.Proteins were consequently extracted from oat brans under normal (CTL) conditions and after pre-treatments of brans with ultrasonic bath (UB), and high power (HP) sonication.There was no significant difference in extraction yields (2.06 -3.79%), meanwhile gel electrophoresis and tandem mass spectrometry (LC-MS/MS) analysis showed differences in polypeptide compositions.Specifically, globulin and avenin proteins were present in all samples, however, ultrasonic treatments (UB and HP) released vromindoline, a starch-bound protein, while tryptophanin, a lipid-bound protein, was released after high power ultrasonic (HP) treatment.The second objective of this work was to determine the physical, chemical, and biological properties of the proteins after hydrolysis with proteases of various specificities.Proteins from CTL, UB, HP samples were hydrolyzed by three proteases (Flavourzyme, Papain, and Alcalase) and then characterized based on their sulfhydryl (SH) content, hydrophobicity and zeta potential.Data showed that HP ultrasonic treatments decreased SH concentrations in Papain and Alcalase hydrolysates but increased it in the Flavourzyme hydrolysate.The surface charge (i.e.zeta potential) was dependent on both the protein extraction and the protease.Papain had a much weaker surface charge (-0.78 to -1.32 mV) than the other proteases (-3.67 to -9.17 mV).UB treatments increased hydrophobicity for Flavourzyme and Papain hydrolysates, while HP treatments decreased the hydrophobicity.To further evaluate the hydrolyzed proteins (i.e.hydrolysates), their biological activities were determined.It was found that in general, ultrasonic treatments reduced the antioxidant activity of the protein hydrolysates.For example, peroxyl radical scavenging activity was reduced by UB and HP sonication; however, HO• scavenging activity was reduced by HP and not UB sonication.In the antidiabetic tests, hydrolysates were evaluated for their ability to modulate the activity of dipeptidyl peptidase-4 (DPP-4) and alpha-amylase, as well as the secretion of GLP-1, a hormone that stimulates insulin response in NCI-H716 cells.Ultrasonication did not affect DPP-4 inhibition; however, concerning proteases, Papain hydrolysates had substantially greater (49.7 -53.6%)DPP-4 inhibitory activity for all conditions.HP sonication slightly decreased the alpha-amylase inhibitory activity by 4.8 -7.2% compared to UB and control.A two-fold increase in hydrolysate concentration resulted in a 13.7 -76% increase in GLP-1 secretion, indicating a dose-dependent
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».