Nanoparticles for photodiagnosis and photodynamic therapy of malignant diseases
Notice bibliographique
Résumé
Photodiagnosis as well as photodynamic therapy (PDT) are based on the use of a photosensitzer (PS) molecule that upon light illumination will either fluoresce or transfer energy to oxygen molecules to create reactive oxygen species that are highly cytotoxic. Since those molecules have a higher affinity towards malignant tissue, they can be used to either indicate the presence of cancer or to destroy it. The main problem with these techniques resides in the relative lack of specific uptake, resulting in false positive diagnosis, excessive tissue destruction and long standing cutaneous sensitization. Incorporation of PS into nanoparticle platforms aims to minimize those side effects, mainly due to the enhanced permeability retention effect and to enhance photodynamic activity due to monomerization of the active compound. Our group is actively investigating photophysical properties of different carrier systems as well as preclinical work to evaluate potential new diagnostic and therapeutic approaches. Liposomal formulations of mTHPC (Foscan®) have been investigated in collaboration with Biolitec AG. The PS was rapidly eliminated from the plasma and maximal therapeutic efficacy was observed when microscopic studies indicated presence of high drug doses in both endothelial cells and tumor cells. Intratumoral drug injections were tested in a model of breast cancer recurrence and revealed unusual features, namely progressive increase of fluorescence that was maximal 24 H after administration. This appeared to be due to a phenomenon of photoinduced fluorescence quenching due to energy transfer between PS molecules within the liposomes. We also developed a new approach for PDT, namely to apply illumination in order to prevent recurrence of bladder cancer following fluorescence guided transurethral resection. In order to avoid excessive photobleaching of the drug, we are currently investigating dendrimerized photosensitizers in collaboration with the University College of London. Those dendrimers appear to have a much higher affinity towards bladder cancer in vivo and to provide a sustained release and uptake of the active component. A third axis of our research is to visualize sentinel lymph nodes with quantum dots. Together with the EPCI (Industrial Physico-Chemical High School, Paris), we have defined the coating that offers the highest lymphotropicity in a newly developed model of metastatic breast cancer. We are currently investigating a new imaging tool to detect thoracic lymph nodes containing NIR emitting quantum dots
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».