SURVIVAL AND PROLIFERATION OF CAMPYLOBACTER IN ALBUMEN, YOLK, AND SHELL MEMBRANES OF BROILER BREEDER HATCHING EGGS
Notice bibliographique
Résumé
Fertile eggs were collected from 47-week-old broiler breeder hens, brought to the laboratory, and separated in 3 segments: albumen, yolk and membranes. Membranes were peeled away from the shell and wiped with 10.0 mL of buffered peptone. Initial pH of albumen and yolk was determined, but no pH was determined for the membranes due to settling of the membrane to the bottom of the tube. Then each segment was stored under refrigeration (4°C), at room temperature (22°C) and in an incubator (37°C) for 2 and 7 d with pH's taken at each time interval. Five replications (individual eggs) were taken. In addition, an inoculum of 3.4 A— 102/mL of a gentamicin-resistant Campylobacter coli was added to each segment of the egg and incubated at 22°C for 3 h; then C. coli was enumerated. Three full replications were conducted. Initial pH was 8.7 and 6.1 for the albumen and yolk. After 48 h at 4°C, 22°C and 37°C, the pH of the albumen, yolk and membrane were 9.0, 9.2, 9.4; 6.3, 7.1, 7.2; 8.2, 8.6, 8.4, respectively. When 2.53 log10 of C. coli was inoculated into albumen, yolk and membrane, the numbers increased in all 3 and were log10 3.0, 2.94, and 2.82, respectively. This demonstrates that Campylobacter not only survives in the different portions of the fertile egg but multiplies slightly, thereby strengthening the argument that Campylobacter may pass from one generation to the next via the fertile egg.Fertile eggs were collected from 47-week-old broiler breeder hens, brought to the laboratory, and separated in 3 segments: albumen, yolk and membranes. Membranes were peeled away from the shell and wiped with 10.0 mL of buffered peptone. Initial pH of albumen and yolk was determined, but no pH was determined for the membranes due to settling of the membrane to the bottom of the tube. Then each segment was stored under refrigeration (4°C), at room temperature (22°C) and in an incubator (37°C) for 2 and 7 d with pH's taken at each time interval. Five replications (individual eggs) were taken. In addition, an inoculum of 3.4 A— 102/mL of a gentamicin-resistant Campylobacter coli was added to each segment of the egg and incubated at 22°C for 3 h; then C. coli was enumerated. Three full replications were conducted. Initial pH was 8.7 and 6.1 for the albumen and yolk. After 48 h at 4°C, 22°C and 37°C, the pH of the albumen, yolk and membrane were 9.0, 9.2, 9.4; 6.3, 7.1, 7.2; 8.2, 8.6, 8.4, respectively. When 2.53 log10 of C. coli was inoculated into albumen, yolk and membrane, the numbers increased in all 3 and were log10 3.0, 2.94, and 2.82, respectively. This demonstrates that Campylobacter not only survives in the different portions of the fertile egg but multiplies slightly, thereby strengthening the argument that Campylobacter may pass from one generation to the next via the fertile egg.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».