Direct inhibition of phosphate transport by immune signaling in Arabidopsis
Notice bibliographique
Résumé
Soil availability of inorganic ortho-phosphate (PO 4 3− , P i ) is a key determinant of plant growth and fitness. 1 1. Bieleski, R.L. Phosphate pools, phosphate transport, and phosphate availability Annu. Rev. Plant Physiol. 1973; 24 :225-252 Crossref Google Scholar Plants regulate the capacity of their roots to take up inorganic phosphate by adapting the abundance of H + -coupled phosphate transporters of the PHOSPHATE TRANSPORTER 1 (PHT1) family 2 2. Nussaume, L. ∙ Kanno, S. ∙ Javot, H. ... Phosphate import in plants: focus on the PHT1 transporters Front. Plant Sci. 2011; 2 :83 Crossref Scopus (423) PubMed Google Scholar at the plasma membrane (PM) through transcriptional and post-translational changes driven by the genetic network of the phosphate starvation response (PSR). 3–8 3. Shin, H. ∙ Shin, H.-S. ∙ Dewbre, G.R. ... Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments Plant J. 2004; 39 :629-642 Crossref Scopus (641) PubMed Google Scholar 4. Misson, J. ∙ Thibaud, M.C. ∙ Bechtold, N. ... Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants Plant Mol. Biol. 2004; 55 :727-741 Crossref Scopus (213) PubMed Google Scholar 5. Thibaud, M.C. ∙ Arrighi, J.F. ∙ Bayle, V. ... Dissection of local and systemic transcriptional responses to phosphate starvation in Arabidopsis Plant J. 2010; 64 :775-789 Crossref Scopus (258) PubMed Google Scholar 6. Bayle, V. ∙ Arrighi, J.-F.F. ∙ Creff, A. ... Arabidopsis thaliana high-affinity phosphate transporters exhibit multiple levels of posttranslational regulation Plant Cell. 2011; 23 :1523-1535 Crossref Scopus (208) PubMed Google Scholar 7. Park, B.S. ∙ Seo, J.S. ∙ Chua, N.H. NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis Plant Cell. 2014; 26 :454-464 Crossref Scopus (213) PubMed Google Scholar 8. Liao, Y.Y. ∙ Li, J.L. ∙ Pan, R.L. ... Structure-function analysis reveals amino acid residues of Arabidopsis phosphate transporter At PHT1;1 crucial for its activity Front. Plant Sci. 2019; 10 :1158 Crossref Scopus (13) PubMed Google Scholar Increasing evidence also shows that plants integrate immune responses to alleviate phosphate starvation stress through the association with beneficial microbes. 9–11 9. Castrillo, G. ∙ Teixeira, P.J.P.L. ∙ Paredes, S.H. ... Root microbiota drive direct integration of phosphate stress and immunity Nature. 2017; 543 :513-518 Crossref Scopus (604) PubMed Google Scholar 10. Finkel, O.M. ∙ Salas-González, I. ∙ Castrillo, G. ... The effects of soil phosphorus content on plant microbiota are driven by the plant phosphate starvation response PLoS Biol. 2019; 17 :e3000534 Crossref Scopus (124) PubMed Google Scholar 11. Shi, J. ∙ Zhao, B. ∙ Zheng, S. ... A phosphate starvation response-centered network regulates mycorrhizal symbiosis Cell. 2021; 184 :5527-5540.e18 Full Text Full Text (PDF) Scopus (168) PubMed Google Scholar Whether and how such phosphate transport is regulated upon activation of immune responses is yet uncharacterized. To address this question, we first developed quantitative assays based on changes in the electrical PM potential to measure active P i transport in roots in real time. By inserting micro-electrodes into bulging root hairs, we were able to determine key characteristics of phosphate transport in intact Arabidopsis thaliana (hereafter Arabidopsis ) seedlings. The fast P i -induced depolarization observed was dependent on the activity of the major phosphate transporter PHT1;4. Notably, we observed that this PHT1;4-mediated phosphate uptake is repressed upon activation of pattern-triggered immunity. This inhibition depended on the receptor-like cytoplasmic kinases BOTRYTIS-INDUCED KINASE 1 (BIK1) and PBS1-LIKE KINASE 1 (PBL1), which both phosphorylated PHT1;4. As a corollary to this negative regulation of phosphate transport by immune signaling, we found that PHT1;4-mediated phosphate uptake normally negatively regulates anti-bacterial immunity in roots. Collectively, our results reveal a mechanism linking plant immunity and phosphate homeostasis, with BIK1/PBL1 providing a molecular integration point between these two important pathways.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction machine sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Le volet Gemma est une étiquette directe du modèle pour chaque travail de la base, lue sur la notice réduite au titre. Le volet Codex est un classifieur appris des 10 348 étiquettes directes de Codex et calibré sur les taux pondérés de l'échantillon; les champs sans appui suffisant ne portent aucun appel Codex. Le mode candidate est l'union des deux volets; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont pas des étiquettes humaines.
Scores du classifieur distillé par catégorie (deux têtes)
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,001 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,001 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,001 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule source (Gemma direct ou Codex distillé), pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».